期刊论文详细信息
Materials
Geometry Effects of Axisymmetric Flow-Focusing Microchannels for Single Cell Encapsulation
Mohammad Nooranidoost1  Ranganathan Kumar1 
[1] Department of Mechanical and Aerospace Engineering, University of Central Florida, Orlando, FL 32816, USA;
关键词: flow-focusing;    cell encapsulation;    microfluidics;    front-tracking method;   
DOI  :  10.3390/ma12172811
来源: DOAJ
【 摘 要 】

Cell microencapsulation is a promising technique to protect living cells in biomedical applications. Microfluidic devices can be utilized to control the production of high-throughput cell-laden droplets. This paper demonstrates the effects of flow-focusing geometry on the droplet size, frequency of droplet generation, and number of cells per droplet. Orifice radius, orifice length, and nozzle-to-orifice distance can significantly influence the flow-field and manipulate droplet formation. This paper analyzes these geometry effects using a numerical front-tracking method for the three fluid phases. It is found that as the orifice radius increases, the drop size and the number of cells in the droplet increase. For a short orifice radius, increasing the orifice length results in the generation of smaller droplets at higher frequency and fewer cells per droplet. On the other hand, for a longer orifice, droplet production is invariant with respect to orifice length. It is also found that shorter distances between the nozzle and the orifice lead to a more controlled and uniform production of droplets. When the nozzle-to-orifice length is increased, the droplet formation becomes non-uniform and unpredictable. Probability charts are plotted with respect to the orifice length and orifice radius, which show that a greater than 50 % probability of single cell encapsulation can be achieved consistently.

【 授权许可】

Unknown   

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