Exploring Antioxidant and Enzymes (A-Amylase and B-Glucosidase) Inhibitory Activity of Morinda lucida and Momordica charantia Leaves from Benin

Foods

Robert Raclea¹  Mihaela Cudălbeanu²  Bianca Furdui²  RodicaMihaela Dinica²  IoanaOtilia Ghinea²  Durand Dah-Nouvlessounon²  SorinMarius Avamescu³  Aly Savadogo⁴  Cheikna Zongo⁴  Farid Baba-Moussa⁵  Michaelle Chokki⁵  Lamine Baba-Moussa⁶ 

[1] Department of Chemistry, Faculty of Natural Sciences, Imperial College London, London SW7 2AZ, UK;Department of Chemistry, Physics and Environment, “Dunarea de Jos” University of Galati, 47 Domneasca Street, 800008 Galati, Romania;Department of Organic Chemistry, Biochemistry and Catalysis, Faculty of Chemistry, University of Bucharest, 90-92 Soseaua Panduri, Bucharest, Romania;Laboratoire de Biochimie et immunologie Appliquées (LABIA), Ecole Doctorale Sciences et Technologies, Université Joseph KI-ZERBO, 03 BP 7021 Ouagadougou 03, Burkina-Faso;Laboratoire de Microbiologie et de Technologie Alimentaire, FAST, Université d’Abomey-Calavi, ISBA-Champ de foire, Cotonou 01BP: 526, Benin;Laboratory of Biology and Molecular Typing in Microbiology, Department of Biochemistry and Cell Biology, University of Abomey-Calavi, Faculty of Sciences and Techniques, Cotonou 05BP1604, Benin;

关键词: antioxidant;    diabetes mellitus;    plant extracts;    HPLC;    DPPH;    β-Carotene–linoleate bleaching;   

DOI  :  10.3390/foods9040434

来源: DOAJ

【 摘 要 】

Background: Momordica charantia Linn. (Cucurbitaceae), the wild variety of bitter melon and Morinda lucida Benth (Rubiaceae) were commonly used as a popular folk medicine in Benin. This research focused to measure the antioxidant and enzyme inhibitory effects of M. charantia and M. lucida leaves and their antidiabetic activity. Methods: Antioxidant activities were evaluated by micro-dilution technique using DPPH free radical scavenging activity and β-carotene-linoleate bleaching assay. The α-amylase inhibition assay was carried out utilizing the 3,5-dinitrosalicylic acid procedure, while β-glucosidase inhibition assay was demonstrated using as substrate p-nitrophenyl-β-D-glucopyranoside (PNPG). HPLC-DAD analysis was realized using a high-performance liquid chromatography systems with diode-array detector, L-3000. Results: Chlorogenic acid, epicatechin, daidzein, rutin, naringin, quercetin, naringenin and genistein were identified as polyphenol compounds in the both plants extract. Dichloromethane and ethyl acetate extracts showed a good α-amylase inhibitory activity (56.46 ± 1.96% and 58.76 ± 2.74% respectively). M. lucida methanolic extract has shown IC₅₀ of 0.51 ± 0.01 mg/mL, which is the lowest for DPPH scavenging activity. M. lucida dichloromethane extract showed the highest inhibitory capacity of β-glucosidase activity (82.11. ± 2.15%). Conclusion: These results justify some traditional medicinal uses of both plants. The purified fractions could be used in future formulations, possibly incorporated in functional foods to combat certain diseases.

【 授权许可】

Unknown