Association between tensin 1 and p130Cas at focal adhesions links actin inward flux to cell migration

Biology Open

Hiroaki Hirata¹  Yasuhiro Sawada¹  Keigo Araki¹  Zhihai Zhao¹  Keiko Kawauchi¹  Song Hui Tan¹  Hiroaki Machiyama¹ 

[1] Mechanobiology Institute, National University of Singapore, 5A Engineering Drive 1, 117411, Singapore;

关键词: Tensin 1;    P130Cas;    Actin cytoskeleton;    Cell migration;    Focal adhesion;   

DOI  :  10.1242/bio.016428

来源: DOAJ

【 摘 要 】

Cell migration is a highly dynamic process that plays pivotal roles in both physiological and pathological processes. We have previously reported that p130Cas supports cell migration through the binding to Src as well as phosphorylation-dependent association with actin retrograde flow at focal adhesions. However, it remains elusive how phosphorylated Cas interacts with actin cytoskeletons. We observe that the actin-binding protein, tensin 1, co-localizes with Cas, but not with its phosphorylation-defective mutant, at focal adhesions in leading regions of migrating cells. While a truncation mutant of tensin 1 that lacks the phosphotyrosine-binding PTB and SH2 domains (tensin 1-SH2PTB) poorly co-localizes or co-immunoprecitates with Cas, bacterially expressed recombinant tensin 1-SH2PTB protein binds to Cas in vitro in a Cas phosphorylation-dependent manner. Furthermore, exogenous expression of tensin 1-SH2PTB, which is devoid of the actin-interacting motifs, interferes with the Cas-driven cell migration, slows down the inward flux of Cas molecules, and impedes the displacement of Cas molecules from focal adhesions. Taken together, our results show that tensin 1 links inwardly moving actin cytoskeletons to phosphorylated Cas at focal adhesions, thereby driving cell migration.

【 授权许可】

Unknown