Vitamin D Receptor Gene Expression in Adipose Tissue of Obese Individuals is Regulated by miRNA and Correlates with the Pro-Inflammatory Cytokine Level

International Journal of Molecular Sciences

Maurycy Jonas¹  Wojciech Lisik¹  Krzysztof Kozniewski²  Alina Kuryłowicz²  MartaIzabela Jonas²  Monika Puzianowska-Kuznicka²  Zbigniew Bartoszewicz² 

[1] Department of General and Transplantation Surgery, Medical University of Warsaw, 00-001 Warsaw, Poland;Department of Human Epigenetics, Mossakowski Medical Research Centre, Polish Academy of Sciences, 5 Pawinskiego Street, 02-106 Warsaw, Poland;

关键词: adipose tissue;    obesity;    vitamin d receptor (vdr);    micro rna (mirna);    pro-inflammatory cytokines;   

DOI  :  10.3390/ijms20215272

来源: DOAJ

【 摘 要 】

Background: Given the role that vitamin D (VD) plays in the regulation of the inflammatory activity of adipocytes, we aimed to assess whether obesity changes the expression of VD-related genes in adipose tissue and, if so, to investigate whether this phenomenon depends on microRNA interference and how it may influence the local inflammatory milieu. Methods: The expression of genes encoding VD 1α-hydroxylase (CYP27B1), 24-hydroxylase (CYP24A1) and receptor (VDR), selected interleukins and microRNAs was evaluated by real-time PCR in visceral (VAT) and in subcutaneous (SAT) adipose tissues of 55 obese (BMI > 40 kg/m²) and 31 normal-weight (BMI 20−24.9 kg/m²) individuals. Results: VDR mRNA levels were higher, while CYP27B1 levels were lower in adipose tissues of obese patients than in those of normal-weight controls (VAT: P = 0.04, SAT: P < 0.0001 and VAT: P = 0.004, SAT: P = 0.016, respectively). The expression of VDR in VAT of obese subjects correlated negatively with levels of miR-125a-5p (P = 0.0006, r_s = −0.525), miR-125b-5p (P = 0.001, r_s = −0.495), and miR-214-3p (P = 0.009, r_s = −0.379). Additionally, VDR mRNA concentrations in visceral adipose tissues of obese subjects correlated positively with mRNA levels of interleukins: 1β, 6 and 8. Conclusions: We observed obesity-associated up-regulation of VDR and down-regulation of CYP27B mRNA levels in adipose tissue. VDR expression correlates with the expression of pro-inflammatory cytokines and may be regulated by miRNAs.

【 授权许可】

Unknown