The genomes of two parasitic wasps that parasitize the diamondback moth

BMC Genomics

Xiaoxiao Hu¹  Hongqing Xie¹  Michael R. Strand²  Francesco Pennacchio³  Fei Li⁴  Jiangyan Zhu⁴  Xiqian Ye⁴  Chuanlin Yin⁴  Yanping Wang⁴  Dianhao Guo⁴  Min Shi⁴  Zehua Wang⁴  Jiani Zou⁴  Yuenan Zhou⁴  Jian Yang⁴  Xuexin Chen⁴  Qijuan Gu⁴  Jianhua Huang⁴  Zhizhi Wang⁴  Leqing Zhan⁴  Rongmin Hu⁴  Shujun Wei⁵  Yuan Yao⁶ 

[1] BGI-Tech, BGI-Shenzhen;Department of Entomology, University of Georgia;Dipartimento di Agraria, Laboratorio di Entomologia “E. Tremblay”, Università di Napoli “Federico II”, Via Università 100;Institute of Insect Sciences, College of Agriculture and Biotechnology, Zhejiang University;Institute of Plant and Environmental Protection, Beijing Academy of Agriculture and Forestry Sciences;School of Physical Science and Technology, Shanghai Tech University;

关键词: Cotesia vestalis;    Diadromus collaris;    Parasitic wasps;    Genome;    Transcriptome;   

DOI  :  10.1186/s12864-019-6266-0

来源: DOAJ

【 摘 要 】

Abstract Background Parasitic insects are well-known biological control agents for arthropod pests worldwide. They are capable of regulating their host’s physiology, development and behaviour. However, many of the molecular mechanisms involved in host-parasitoid interaction remain unknown. Results We sequenced the genomes of two parasitic wasps (Cotesia vestalis, and Diadromus collaris) that parasitize the diamondback moth Plutella xylostella using Illumina and Pacbio sequencing platforms. Genome assembly using SOAPdenovo produced a 178 Mb draft genome for C. vestalis and a 399 Mb draft genome for D. collaris. A total set that contained 11,278 and 15,328 protein-coding genes for C. vestalis and D. collaris, respectively, were predicted using evidence (homology-based and transcriptome-based) and de novo prediction methodology. Phylogenetic analysis showed that the braconid C. vestalis and the ichneumonid D. collaris diverged approximately 124 million years ago. These two wasps exhibit gene gains and losses that in some cases reflect their shared life history as parasitic wasps and in other cases are unique to particular species. Gene families with functions in development, nutrient acquisition from hosts, and metabolism have expanded in each wasp species, while genes required for biosynthesis of some amino acids and steroids have been lost, since these nutrients can be directly obtained from the host. Both wasp species encode a relative higher number of neprilysins (NEPs) thus far reported in arthropod genomes while several genes encoding immune-related proteins and detoxification enzymes were lost in both wasp genomes. Conclusions We present the annotated genome sequence of two parasitic wasps C. vestalis and D. collaris, which parasitize a common host, the diamondback moth, P. xylostella. These data will provide a fundamental source for studying the mechanism of host control and will be used in parasitoid comparative genomics to study the origin and diversification of the parasitic lifestyle.

【 授权许可】

Unknown