Single nucleotide polymorphisms in CXCR1 gene and its association with hepatitis B infected patients in Saudi Arabia

Annals of Hepatology

Mohammed Al-Ahdal¹  Hind Al-Humaidan²  Mashael Al-Anazi³  Hamad Al-Ashgar⁴  Faisal M. Sanai⁵  Nisreen Khalaf⁵  Majid Al-Okail⁵  Zahid Hussain⁵  Fahad N. Almajhdi⁶  Riham Al-Swayeh⁷  Saud Alarifi⁷  Ayman A. Abdo⁷  Ahmed Al-Qahtani⁷  Nisha A. Viswan⁸  Khalid Al-Kahtani⁹ 

[1] Center of Excellence in Biotechnology Research, King Saud University, Riyadh, Saudi Arabia;Department of Hepatobiliary Science and Liver Transplantation, King Abdulaziz Medical City, Riyadh, Saudi Arabia;;Department of Pathology and Laboratory Medicine, King Faisal Specialist Hospital &Liver Disease Research Center, King Saud University, Riyadh, Saudi Arabia;Research Center, Riyadh, Saudi Arabia;Department of Botany and Microbiology, College of Science, King Saud University, Riyadh, Saudi Arabia;;Department of Infection and Immunity, Research Center, King Faisal Specialist Hospital &Department of Medicine, College of Medicine, King Saud University, Riyadh, Saudi Arabia;Liver Disease Research Center, King Saud University, Riyadh, Saudi Arabia;

关键词: Hepatitis B Virus;    CXCR1;    Single nucleotide polymorphisms;    Variations;    Genetics;    Haplotype;   

DOI  :  

来源: DOAJ

【 摘 要 】

Background/Aim. This study aims to investigate whether the SNPs of CXCR1 gene, could predict the likelihood of viral persistence and/or disease progression.Material and methods. We investigated the association of two different SNPs (rs2234671, and rs142978743) in 598 normal healthy controls and 662 HBV patients from a Saudi ethnic population. The HBV patients were categorized into inactive carriers (n = 428), active carriers (n = 162), cirrhosis (n = 54) and Cirrhosis-HCC (n = 18) sub-groups. Genetic variants in CXCR1 were determined by polymerase chain reaction (PCR)-based DNA direct sequencing.Results. The frequency of the risk allele ‘C’ for the SNP, rs2234671 was found to be insignificant when the patient group was compared to the uninfected control group, however, a significant distribution of the allele ‘C’ of rs2234671 was observed among active HBV carriers + cirrhosis + cirrhosis - HCC vs. inactive HBV carriers with an OR = 1.631 (95% C.I. 1.016-2.616) and p = 0.032. However, no significant association was observed for rs142978743 when the distribution of risk allele was analyzed among the different patient groups (i.e. inactive carriers, active carriers, cirrhosis and HCC). Furthermore, the most common haplotype, Haplo-1 (AG), was found to have an insignificant frequency distribution between HBV cases and controls, while the same haplotype was found to be significantly distributed when active carriers + cirrhosis + cirrhosis - HCC patients were compared to inactive HBV carriers with a frequency of 0.938 and p = 0.0315. Haplo-2 (AC) was also found to be significantly associated with a frequency of 0.058 and p = 0.0163.Conclusion. The CXCR1 polymorphism, rs2234671 was found to be associated with chronic HBV infection and may play a role in disease activity.

【 授权许可】

Unknown