S. aureus Biofilm Protein Expression Linked to Antimicrobial Resistance: A Proteomic Study

Animals

Adriana Ianieri¹  Andrea Urbani²  Viviana Greco²  Nicola Costanzo³  Carlotta Ceniti³  Bruno Tilocca³  Cristian Piras³  Paola Roncada³  PierluigiAldo Di Ciccio⁴  Alessio Soggiu⁵  Luigi Bonizzi⁵ 

[1] Deparment of Food and Drug, University of Parma, Parco Area delle Scienze 27A, 43124 Parma, Italy;Department of Basic Biotechnological Sciences, Intensivological and Perioperative Clinics, Università Cattolica del Sacro Cuore, Largo Francesco Vito 1, 00168 Roma, Italy;Department of Health Sciences, University “Magna Græcia” of Catanzaro, Campus Universitario “S. Venuta”, Viale Europa, I-88100 Catanzaro, Italy;Department of Veterinary Sciences, University of Torino, Largo Paolo Braccini 2, Grugliasco, 10095 Torino, Italy;Surgical and Dental Sciences-One Health Unit, Department of Biomedical, University of Milano, Via Celoria 10, 20133 Milano, Italy;

关键词: Staphylococcus aureus;    planktonic cells;    biofilm;    proteomics;    food safety;    antimicrobial resistance;   

DOI  :  10.3390/ani11040966

来源: DOAJ

【 摘 要 】

Antimicrobial resistance (AMR) represents one of the most critical challenges that humanity will face in the following years. In this context, a “One Health” approach with an integrated multidisciplinary effort involving humans, animals and their surrounding environment is needed to tackle the spread of AMR. One of the most common ways for bacteria to live is to adhere to surfaces and form biofilms. Staphylococcus aureus (S. aureus) can form biofilm on most surfaces and in a wide heterogeneity of environmental conditions. The biofilm guarantees the survival of the S. aureus in harsh environmental conditions and represents an issue for the food industry and animal production. The identification and characterization of biofilm-related proteins may provide interesting insights into biofilm formation mechanisms in S. aureus. In this regard, the aims of this study were: (i) to use proteomics to compare proteomes of S. aureus growing in planktonic and biofilm forms in order to investigate the common features of biofilm formation properties of different strains; (ii) to identify specific biofilm mechanisms that may be involved in AMR. The proteomic analysis showed 14 differentially expressed proteins among biofilm and planktonic forms of S. aureus. Moreover, three proteins, such as alcohol dehydrogenase, ATP-dependent 6-phosphofructokinase, and fructose-bisphosphate aldolase, were only differentially expressed in strains classified as high biofilm producers. Differentially regulated catabolites metabolisms and the switch to lower oxygen-related metabolisms were related to the sessile conformation analyzed.

【 授权许可】

Unknown