In Vitro Newly Isolated Environmental Phage Activity against Biofilms Preformed by Pseudomonas aeruginosa from Patients with Cystic Fibrosis

Microorganisms

AndreaDi Giulio¹  Arianna Pompilio¹  Paola Rosati²  ErsiliaVita Fiscarelli³  Martina Rossitto³  Nour Essa³  Valentina Crocetta⁴  Giovanni Di Bonaventura⁴  Veronica Lupetti⁵ 

[1] Center for Advanced Studies and Technology (CAST), “Gabriele d’Annunzio” University of Chieti-Pescara, 66100 Chieti, Italy;Clinical Pathways and Epidemiology, Bambino Gesù Children’s Hospital OBG, 00165 Rome, Italy;Cystic Fibrosis Diagnostics, Microbiology and Immunology Diagnostics, Bambino Gesù Children’s Hospital (OBG), 00165 Rome, Italy;Department of Medical, Oral and Biotechnological Sciences;Department of Science, Interdepartmental Laboratory of Electron Microscopy, L.I.M.E., Roma Tre University, 00146 Rome, Italy;

关键词: newly isolated environmental phages;    bacteriophage in vitro activity;    phage efficacy;    Pseudomonas aeruginosa;    biofilm;    planktonic cells;   

DOI  :  10.3390/microorganisms9030478

来源: DOAJ

【 摘 要 】

As disease worsens in patients with cystic fibrosis (CF), Pseudomonas aeruginosa (PA) colonizes the lungs, causing pulmonary failure and mortality. Progressively, PA forms typical biofilms, and antibiotic treatments determine multidrug-resistant (MDR) PA strains. To advance new therapies against MDR PA, research has reappraised bacteriophages (phages), viruses naturally infecting bacteria. Because few in vitro studies have tested phages on CF PA biofilms, general reliability remains unclear. This study aimed to test in vitro newly isolated environmental phage activity against PA isolates from patients with CF at Bambino Gesù Children’s Hospital (OBG), Rome, Italy. After testing in vitro phage activities, we combined phages with amikacin, meropenem, and tobramycin against CF PA pre-formed biofilms. We also investigated new emerging morphotypes and bacterial regrowth. We obtained 22 newly isolated phages from various environments, including OBG. In about 94% of 32 CF PA isolates tested, these phages showed in vitro PA lysis. Despite poor efficacy against chronic CF PA, five selected-lytic-phages (Φ4_ZP1, Φ9_ZP2, Φ14_OBG, Φ17_OBG, and Φ19_OBG) showed wide host activity. The Φ4_ZP1-meropenem and Φ14_OBG-tobramycin combinations significantly reduced CF PA biofilms (p < 0.001). To advance potential combined phage-antibiotic therapy, we envisage further in vitro test combinations with newly isolated phages, including those from hospital environments, against CF PA biofilms from early and chronic infections.

【 授权许可】

Unknown