Baicalin Represses Type Three Secretion System of Pseudomonas aeruginosa through PQS System

Molecules

Qiao Guo¹  Kangmin Duan²  Zhihua Wei³  Lin Chen³  Lixin Shen³  Qin Yang³  Zisheng Guo³  Pansong Zhang³ 

[1] College of Natural Resources and Environment, Northwest A&F University, Yangling 712100, China;Department of Oral Biology & Medical Microbiology & Infectious Diseases, Rady Faculty of Health Sciences, University of Manitoba, 780 Bannatyne Ave., Winnipeg, MB R3E 0W2, Canada;Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, Northwest University, Xi’an 710069, China;

关键词: Pseudomonas aeruginosa;    virulence factors;    baicalin;    Type III secretion system;    PQS inhibition;    mouse lung infection model;   

DOI  :  10.3390/molecules26061497

来源: DOAJ

【 摘 要 】

Therapeutics that target the virulence of pathogens rather than their viability offer a promising alternative for treating infectious diseases and circumventing antibiotic resistance. In this study, we searched for anti-virulence compounds against Pseudomonas aeruginosa from Chinese herbs and investigated baicalin from Scutellariae radix as such an active anti-virulence compound. The effect of baicalin on a range of important virulence factors in P. aeruginosa was assessed using luxCDABE-based reporters and by phenotypical assays. The molecular mechanism of the virulence inhibition by baicalin was investigated using genetic approaches. The impact of baicalin on P. aeruginosa pathogenicity was evaluated by both in vitro assays and in vivo animal models. The results show that baicalin diminished a plenty of important virulence factors in P. aeruginosa, including the Type III secretion system (T3SS). Baicalin treatment reduced the cellular toxicity of P. aeruginosa on the mammalian cells and attenuated in vivo pathogenicity in a Drosophila melanogaster infection model. In a rat pulmonary infection model, baicalin significantly reduced the severity of lung pathology and accelerated lung bacterial clearance. The PqsR of the Pseudomonas quinolone signal (PQS) system was found to be required for baicalin’s impact on T3SS. These findings indicate that baicalin is a promising therapeutic candidate for treating P. aeruginosa infections.

【 授权许可】

Unknown