Identification of QTLs/Defense Genes Effective at Seedling Stage Against Prevailing Races of Wheat Stripe Rust in India

Frontiers in Genetics

Anil Rai¹  Neeraj Budhlakoti¹  Dwijesh C. Mishra¹  Monendra Grover¹  Arun Gupta²  Subhash C. Bhardwaj³  Om P. Gangwar³  Subodh Kumar³  Suneel Kumar⁴  Sundeep Kumar⁴  Amit Kumar Singh⁴  Kuldeep Singh⁴  Divya Chauhan⁴  Anjan Kumar Pradhan⁴  Shikha Mittal⁴ 

[1] Indian Council of Agricultural Research-Indian Agricultural Statistics Research Institute, New Delhi, India;Indian Council of Agricultural Research-Indian Institute of Wheat and Barley Research, Karnal, India;Indian Council of Agricultural Research-Indian Institute of Wheat and Barley Research, Regional Station, Shimla, India;Indian Council of Agricultural Research-National Bureau of Plant Genetic Resources, New Delhi, India;

关键词: stripe rust;    wheat;    SNP;    GWAS;    GAPIT;   

DOI  :  10.3389/fgene.2020.572975

来源: DOAJ

【 摘 要 】

Resistance in modern wheat cultivars for stripe rust is not long lasting due to the narrow genetic base and periodical evolution of new pathogenic races. Though nearly 83 Yr genes conferring resistance to stripe rust have been cataloged so far, few of them have been mapped and utilized in breeding programs. Characterization of wheat germplasm for novel sources of resistance and their incorporation into elite cultivars is required to achieve durable resistance and thus to minimize the yield losses. Here, a genome-wide association study (GWAS) was performed on a set of 391 germplasm lines with the aim to identify quantitative trait loci (QTL) using 35K Axiom® array. Phenotypic evaluation disease severity against four stripe rust pathotypes, i.e., 46S119, 110S119, 238S119, and 47S103 (T) at the seedling stage in a greenhouse providing optimal conditions was carried out consecutively for 2 years (2018 and 2019 winter season). We identified, a total of 17 promising QTl which passed FDR criteria. Moreover these 17 QTL identified in the current study were mapped at different genomic locations i.e. 1B, 2A, 2B, 2D, 3A, 3B, 3D, 4B, 5B and 6B. These 17 QTLs identified in the present study might play a key role in marker-assisted breeding for developing stripe rust resistant wheat cultivars.

【 授权许可】

Unknown