Genome-Wide Association Study Identifies Two Loci for Stripe Rust Resistance in a Durum Wheat Panel from Iran

Applied Sciences

Ali Ashraf Mehrabi¹  Mahbubjon Rahmatov²  Oadi Matny³  Brian J. Steffenson³  Alireza Pour-Aboughadareh⁴ 

[1] Department of Plant Biotechnology, Research Institute of Forests and Rangelands, Agricultural Research, Education and Extension Organization (AREEO), Tehran 14968-13111, Iran;Department of Plant Breeding, Swedish University of Agricultural Sciences, Alnarp, 234 22 Lomma, Sweden;Department of Plant Pathology, University of Minnesota, St. Paul, MN 55108, USA;Seed and Plant Improvement Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj 31587-77871, Iran;

关键词: durum wheat;    stripe rust;    Pstv-37 and Pstv-40 isolates;    GWAS;    SNP markers;   

DOI  :  10.3390/app12104963

来源: DOAJ

【 摘 要 】

Stripe rust (Puccinia striiformis f. sp. tritici (Pst)) is one of the most devastating fungal diseases of durum wheat (Triticum turgidum L. var. durum Desf.). Races of Pst with new virulence combinations are emerging more regularly on wheat-growing continents, which challenges wheat breeding for resistance. This study aimed to identify and characterize resistance to Pst races based on a genome-wide association study. GWAS is an approach to analyze the associations between a genome-wide set of single-nucleotide polymorphisms (SNPs) and target phenotypic traits. A total of 139 durum wheat accessions from Iran were evaluated at the seedling stage against isolates Pstv-37 and Pstv-40 of Pst and then genotyped using a 15K SNP chip. In total, 230 significant associations were identified across 14 chromosomes, of which 30 were associated with resistance to both isolates. Furthermore, 17 durum wheat landraces showed an immune response against both Pst isolates. The SNP markers and resistant accessions identified in this study may be useful in programs breeding durum wheat for stripe rust resistance.

【 授权许可】

Unknown