Comodulation of NO-Dependent Vasodilation by Erythroid Band 3 and Hemoglobin: A GP.Mur Athlete Study

Frontiers in Cardiovascular Medicine

Hung-I Yeh¹  Kuang-Tse Huang²  Yen-Yu Liu³  Kuo-Wei Tseng⁴  Hui-Ju Lin⁵  Hui-Lin Lee⁵  Li-Yang Chen⁵  Chia-Yuan Liu⁶  Wei-Chin Tseng⁷  Kate Hsu⁹ 

[1] 0Division of Cardiology, Departments of Internal Medicine, MacKay Memorial Hospital, Taipei, Taiwan;Department of Chemical Engineering, National Chung-Cheng University, Chia-Yi, Taiwan;Department of Critical Care Medicine, MacKay Memorial Hospital, New Taipei City, Taiwan;Department of Exercise and Health Sciences, University of Taipei, Taipei, Taiwan;Department of Medical Research, MacKay Memorial Hospital, New Taipei City, Taiwan;Department of Medicine, MacKay Medical College, New Taipei City, Taiwan;Department of Physical Education, University of Taipei, Taipei, Taiwan;Division of Gastroenterology, Department of Internal Medicine, MacKay Memorial Hospital, Taipei, Taiwan;Institute of Biomedical Sciences, Mackay Medical College, New Taipei City, Taiwan;MacKay Junior College of Medicine, Nursing, and Management, New Taipei City, Taiwan;

关键词: flow-mediated vasodilation (FMD);    nitric oxide (NO);    Band 3;    GP.Mur;    red blood cell (RBC);    hemoglobin (Hb);   

DOI  :  10.3389/fcvm.2021.740100

来源: DOAJ

【 摘 要 】

GP.Mur, a red blood cell (RBC) hybrid protein encoded by glycophorin B-A-B, increases expression of erythroid band 3 (Anion Exchanger-1, SLC4A1). GP.Mur is extremely rare but has a prevalence of 1–10% in regions of Southeast Asia. We unexpectedly found slightly higher blood pressure (BP) among healthy Taiwanese adults with GP.Mur. Since band 3 has been suggested to interact with hemoglobin (Hb) to modulate nitric oxide (NO)-dependent hypoxic vasodilation during the respiratory cycle, we hypothesized that GP.Mur red cells could exert differentiable effects on vascular tone. Here we recruited GP.Mur-positive and GP.Mur-negative elite male college athletes, as well as age-matched, GP.Mur-negative non-athletes, for NO-dependent flow-mediated dilation (FMD) and NO-independent dilation (NID). The subjects were also tested for plasma nitrite and nitrate before and after arterial occlusion in FMD. GP.Mur+ and non-GP.Mur athletes exhibited similar heart rates and blood pressure, but GP.Mur+ athletes showed significantly lower FMD (4.8 ± 2.4%) than non-GP.Mur athletes (6.5 ± 2.1%). NO-independent vasodilation was not affected by GP.Mur. As Hb controls intravascular NO bioavailability, we examined the effect of Hb on limiting FMD and found it to be significantly stronger in GP.Mur+ subjects. Biochemically, plasma nitrite levels were directly proportional to individual band 3 expression on the red cell membrane. The increase of plasma nitrite triggered by arterial occlusion also showed small dependency on band 3 levels in non-GP.Mur subjects. By the GP.Mur comparative study, we unveiled comodulation of NO-dependent vasodilation by band 3 and Hb, and verified the long-pending role of erythroid band 3 in this process.

【 授权许可】

Unknown