FEBS Letters | |
The expression of the abnormal human red cell anion transporter from South‐East Asian ovalocytes (band 3 SAO) in Xenopus oocytes | |
Groves, Jonathan D.1  Tanner, Michael J.A.1  Ring, Susan M.1  Schofield, Ann E.1  | |
[1] Department of Biochemistry, School of Medical Sciences, University of Bristol, Bristol BS8 1TD, UK | |
关键词: Hereditary ovalocytosis; Band 3; Glycophorin A; Anion transporter; Xenopus oocyte; BADS; 4-benzamido-4'-aminostilbene-2; 2'-disulphonate; DIDS; 4; 4'-diisothiocyanato-2; 2'-stilbene disulphonate; DNDS; 4; 4'-dinitrostilbene-2; 2'-disulphonate; GPA; glycophorin A; SAO; South-East Asian hereditary ovalocytosis; | |
DOI : 10.1016/0014-5793(93)80270-5 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
South-East Asian ovalocytosis (SAO) is caused by the heterozygous presence of a variant form of the human erythrocyte anion transporter (band 3; AE1). The expression of band 3 SAO has been studied in Xenopus oocytes. Band 3 SAO is not functional as an anion transporter but is inserted stably into the plasma membrane of oocytes. Band 3 SAO translocation to the cell surface does not require co-expression of normal band 3. Co-expression of glycophorin A (GPA) increases the rate of translocation of band 3 SAO to the oocyte membrane but is not essential for this process. We suggest that the increased tendency of band 3 SAO to form oligomers may facilitate its translocation to the cell surface.
【 授权许可】
Unknown
【 预 览 】
Files | Size | Format | View |
---|---|---|---|
RO201912020298414ZK.pdf | 568KB | download |