The expression of the abnormal human red cell anion transporter from South‐East Asian ovalocytes (band 3 SAO) in Xenopus oocytes

FEBS Letters

Groves, Jonathan D.¹  Tanner, Michael J.A.¹  Ring, Susan M.¹  Schofield, Ann E.¹ 

[1] Department of Biochemistry, School of Medical Sciences, University of Bristol, Bristol BS8 1TD, UK

关键词: Hereditary ovalocytosis;    Band 3;    Glycophorin A;    Anion transporter;    Xenopus oocyte;    BADS;    4-benzamido-4'-aminostilbene-2;    2'-disulphonate;    DIDS;    4;    4'-diisothiocyanato-2;    2'-stilbene disulphonate;    DNDS;    4;    4'-dinitrostilbene-2;    2'-disulphonate;    GPA;    glycophorin A;    SAO;    South-East Asian hereditary ovalocytosis;   

DOI  :  10.1016/0014-5793(93)80270-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

South-East Asian ovalocytosis (SAO) is caused by the heterozygous presence of a variant form of the human erythrocyte anion transporter (band 3; AE1). The expression of band 3 SAO has been studied in Xenopus oocytes. Band 3 SAO is not functional as an anion transporter but is inserted stably into the plasma membrane of oocytes. Band 3 SAO translocation to the cell surface does not require co-expression of normal band 3. Co-expression of glycophorin A (GPA) increases the rate of translocation of band 3 SAO to the oocyte membrane but is not essential for this process. We suggest that the increased tendency of band 3 SAO to form oligomers may facilitate its translocation to the cell surface.

【 授权许可】

Unknown