Transcriptome Analysis of the Hierarchical Response of Histone Deacetylase Proteins That Respond in an Antagonistic Manner to Salinity Stress

Frontiers in Plant Science

Shunsuke Watanabe²  Mitsunori Seo²  Miyako Kusano³  Kazuki Saito⁴  Motoaki Seki⁵  Makoto Kobayashi⁶  Minoru Ueda⁷  Akihiro Matsui⁷  Junko Ishida⁷  Maho Tanaka⁷ 

[1] Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST), Kawaguchi, Japan;Dormancy and Adaptation Research Unit, RIKEN Center for Sustainable Resource Science, Yokohama, Japan;Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Japan;Graduate School of Pharmaceutical Sciences, Chiba University, Chiba, Japan;Kihara Institute for Biological Research, Yokohama City University, Yokohama, Japan;Metabolomics Research Group, RIKEN Center for Sustainable Resource Science, Yokohama, Japan;Plant Epigenome Regulation Laboratory, RIKEN Cluster for Pioneering Research, Wako, Japan;Plant Genomic Network Research Team, RIKEN Center for Sustainable Resource Science, Yokohama, Japan;

关键词: high salinity stress;    epigenetics;    histone acetylation;    histone deacetylases;    stress response;   

DOI  :  10.3389/fpls.2019.01323

来源: DOAJ

【 摘 要 】

Acetylation in histone and non-histone proteins is balanced by histone acetyltransferase and histone deacetylase (HDAC) enzymatic activity, an essential aspect of fine-tuning plant response to environmental stresses. HDACs in Arabidopsis are composed of three families (RPD3-like, SIRT, and HD-tuins). A previous study indicated that class I (HDA19) and class II (HDA5/14/15/18) RPD3-like family HDACs control positive and negative responses to salinity stress, respectively. Furthermore, quintuple hda5/14/15/18/19 mutants (quint) exhibit salinity stress tolerance, suggesting that hda19 suppresses the sensitivity to salinity stress present in quadruple hda5/14/15/18 mutants (quad). In the present study, transcriptome analysis of the quint mutant was conducted to elucidate the hierarchical control of salinity stress response operated by RPD3-like family HDACs (HDA5/14/15/18/19). The analysis identified 4,832 salt-responsive genes in wild-type (Col-0), hda19-3, quad, and quint plants and revealed that 56.7% of the salt-responsive genes exhibited a similar expression pattern in both the hda19-3 and quint plants. These results indicate that deficiency in HDA19 has a bigger impact on salinity stress response than in class II HDACs. Furthermore, the expression pattern of genes encoding enzymes that metabolize phytohormones raises the possibility that a drastic change in the homeostasis of phytohormones, such as abscisic acid, brassinosteroid, and gibberellin, may contribute to increasing stress tolerance in hda19-3 and quint plants. Among these phytohormones, abscisic acid accumulation actually increased in hda19-3 and quint plants, and decreased in quad, compared with wild-type plants. Importantly, 7.8% of the salt-responsive genes in quint plants exhibited a similar expression pattern in quad plants, suggesting that some gene sets are regulated in an HDA5/14/15/18-dependent manner. The transcriptome analysis conducted in the present study revealed the hierarchical and independent regulation of salt stress response that is mediated through HDA19 and class II HDACs.

【 授权许可】

Unknown