The Antimicrobial and Anti-Inflammatory Effects of Silver Nanoparticles Synthesised from Cotyledon orbiculata Aqueous Extract

Nanomaterials

Abdulrahman Mohammed Elbagory¹  Mervin Meyer²  Abram Madimabe Madiehe²  Samantha Meyer³  Caroline Tyavambiza³ 

[1] Chemistry Department, Cape Peninsula University of Technology, P.O Box 1906, Bellville, Cape Town 7535, South Africa;DSI/Mintek Nanotechnology Innovation Centre, Department of Biotechnology, University of the Western Cape, Private Bag X17, Bellville, Cape Town 7535, South Africa;Department of Biomedical Sciences, Cape Peninsula University of Technology, P.O. Box 1906, Bellville, Cape Town 7535, South Africa;

关键词: green nanotechnology;    Cotyledon orbiculata;    silver nanoparticles;    nanoparticles characterisation;    antimicrobial activity;    immunomodulation;   

DOI  :  10.3390/nano11051343

来源: DOAJ

【 摘 要 】

Cotyledon orbiculata, commonly known as pig’s ear, is an important medicinal plant of South Africa. It is used in traditional medicine to treat many ailments, including skin eruptions, abscesses, inflammation, boils and acne. Many plants have been used to synthesize metallic nanoparticles, particularly silver nanoparticles (AgNPs). However, the synthesis of AgNPs from C. orbiculata has never been reported before. The aim of this study was to synthesize AgNPs using C. orbiculata and evaluate their antimicrobial and immunomodulatory properties. AgNPs were synthesized and characterized using Ultraviolet-Visible Spectroscopy (UV-Vis), Dynamic Light Scattering (DLS) and High-Resolution Transmission Electron Microscopy (HR-TEM). The antimicrobial activities of the nanoparticles against skin pathogens (Staphylococcus aureus, Staphylococcus epidermidis, Methicillin Resistance Staphylococcus aureus, Pseudomonas aeruginosa and Candida albicans) as well as their effects on cytokine production in macrophages (differentiated from THP-1 cells) were evaluated. The AgNPs from C. orbiculata exhibited antimicrobial activity, with the highest activity observed against P. aeruginosa (5 µg/mL). The AgNPs also showed anti-inflammatory activity by inhibiting the secretion of pro-inflammatory cytokines (TNF-alpha, IL-6 and IL-1 beta) in lipopolysaccharide-treated macrophages. This concludes that the AgNPs produced from C. orbiculata possess antimicrobial and anti-inflammation properties.

【 授权许可】

Unknown