Molecular epidemiology of group A human rotaviruses in North West region, Cameroon

The Pan African Medical Journal

Jarlath Udoudo Umoh¹  Aliyu Ahmadu Ahmad¹  Florence Azie Mbuh¹  George Enyimah Armah²  Sunday Aremu Omilabu³ 

[1] Ahmadu Bello University Zaria, Nigeria;Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Ghana;University of Lagos, Lagos, Nigeria;

关键词: rotavirus;    diarrhea;    molecular epidemiology;    electropherotypes;   

DOI  :  10.11604/pamj.2012.12.108.1698

来源: DOAJ

【 摘 要 】

INTRODUCTION: Rotavirus (RV) is the most common cause of severe diarrhea in children less than 5 years of age worldwide accounting for 527,000 deaths annually. Over 80% of these deaths occur in South Asia and sub-Saharan Africa. RV vaccines have significantly reduced RV-associated morbidity and mortalities in several countries like the United States and Mexico while vaccine trials have proved efficacious in Ghana and other developing countries. However, there is paucity of data on RV infection in Cameroon where diarrhea is a major childhood disease. METHODS: A total of 534 stool specimens collected between January 2003 and December 2004 from children with acute gastroenteritis in five health districts in the NWR of Cameroon were screened for group Ahuman rotavirus antigen by ELISA and their electropherotypes determined by Polyacrylamide gel electrophoresis. RESULTS: RV was detected in 153 (28.7%) diarrheic specimens with infection occurring throughout the year, being more common in children under two years of age (P less than 0.01) with the highest incidence in the 7-9 months age group (P less than0.05). Sub clinical infections (9%) occurred mostly in children aged 0 - 6 months old (Pless than0.01).Source of drinking water was not associated with RV infection. Eleven electropherotype patterns were detected with predominance of long electropherotypes (92.8%) and mixed electropherotypes were seen only in hospitalized children. Some isolates showed overlapping or merged genome segments 7 and 8 or 9 and presenting with 10 segments of the RV genome. CONCLUSION: RV is a significant cause of pediatric diarrhea in the NWR affecting mostly children under 2 years of age. Continuous RV surveillance and nationwidesurveys are recommended to improve the health of young children in Cameroon.More research is needed to fully characterize the isolated RV strains.

【 授权许可】

Unknown