Proteomics and Phosphoproteomics of Heat Stress-Responsive Mechanisms in Spinach

Frontiers in Plant Science

Jiaying Chen¹  Xiaoli Wang¹  Quanhua Wang¹  Chenxi Xu¹  Xiaofeng Cai¹  Wenxin Chen¹  Jiayi Bian¹  Qi Zhao²  Siyi Guo³  Ying Li⁴  Shaojun Dai⁴  Zhiqiang Zhou⁴  Hao Xie⁴  Sixue Chen⁵  Yimin She⁶  Jun Ma⁶ 

[1]Development Center of Plant Germplasm Resources, College of Life and Environmental Sciences, Shanghai Normal University, Shanghai, China

[2]Institute of Life Sciences, Chongqing Medical University, Chongqing, China

[3]Institute of Plant Stress Biology, State Key Laboratory of Cotton Biology, Department of Biology, Henan University, Kaifeng, China

[4]Key Laboratory of Forest Plant Ecology, Key Laboratory of Saline-alkali Vegetation Ecology Restoration, Ministry of Education, Alkali Soil Natural Environmental Science Center, Northeast Forestry University, Harbin, China

[5]Plant Molecular and Cellular Biology Program, Department of Biology, Genetics Institute, Interdisciplinary Center for Biotechnology Research, University of Florida, Gainesville, FL, United States

[6]Shanghai Center for Plant Stress Biology, Chinese Academy of Sciences, Shanghai, China

关键词: heat adaptation;    signal transduction;    ROS homeostasis;    spinach;    proteomics;   

DOI  :  10.3389/fpls.2018.00800

来源: DOAJ

【 摘 要 】

Elevated temperatures limit plant growth and reproduction and pose a growing threat to agriculture. Plant heat stress response is highly conserved and fine-tuned in multiple pathways. Spinach (Spinacia oleracea L.) is a cold tolerant but heat sensitive green leafy vegetable. In this study, heat adaptation mechanisms in a spinach sibling inbred heat-tolerant line Sp75 were investigated using physiological, proteomic, and phosphoproteomic approaches. The abundance patterns of 911 heat stress-responsive proteins, and phosphorylation level changes of 45 phosphoproteins indicated heat-induced calcium-mediated signaling, ROS homeostasis, endomembrane trafficking, and cross-membrane transport pathways, as well as more than 15 transcription regulation factors. Although photosynthesis was inhibited, diverse primary and secondary metabolic pathways were employed for defense against heat stress, such as glycolysis, pentose phosphate pathway, amino acid metabolism, fatty acid metabolism, nucleotide metabolism, vitamin metabolism, and isoprenoid biosynthesis. These data constitute a heat stress-responsive metabolic atlas in spinach, which will springboard further investigations into the sophisticated molecular mechanisms of plant heat adaptation and inform spinach molecular breeding initiatives.

【 授权许可】

Unknown