UBE2C is a Potential Biomarker for Tumorigenesis and Prognosis in Tongue Squamous Cell Carcinoma

Diagnostics

Chun-Lin Chen¹  Pei-Feng Liu²  Cheng-Hsin Lee²  Huei-Han Liou³  Luo-Ping Ger³  Hui-Min Chang³  Bor-Hwang Kang⁴  Chun-Feng Chen⁵  Chih-Wen Shu⁶ 

[1] Department of Biological Sciences, National Sun Yat-sen University, Kaohsiung 80424, Taiwan;Department of Biomedical Science and Environmental Biology, Kaohsiung Medical University, Kaohsiung 80756, Taiwan;Department of Medical Education and Research, Kaohsiung Veterans General Hospital, Kaohsiung 81362, Taiwan;Department of Otorhinolaryngology-Head and Neck Surgery, Kaohsiung Veterans General Hospital, Kaohsiung 81362, Taiwan;Department of Stomatology, Kaohsiung Veterans General Hospital, Kaohsiung 81362, Taiwan;Institute of Biomedical Sciences, National Sun Yat-sen University, Kaohsiung 80424, Taiwan;

关键词: UBE2C;    tongue squamous cell carcinoma;    tumorigenesis;    prognosis;    biomarker;   

DOI  :  10.3390/diagnostics10090674

来源: DOAJ

【 摘 要 】

Ubiquitin-conjugating enzyme 2C (UBE2C) involves in numerous cellular processes and the tumor progression in many cancers. However, its role in oral squamous cell carcinoma (OSCC) is unclear. We aimed to investigate the role and clinical significance of UBE2C in OSCC. The expression levels of UBE2C were examined by immunohistochemistry in 185 buccal mucosa squamous cell carcinomas, 247 tongue squamous cell carcinomas (TSCCs) and 75 lip squamous cell carcinomas. The roles of UBE2C in cell growth, invasion/migration and cancer stemness were also examined in OSCC cells. The expression levels of UBE2C protein were higher in tumor tissues than they were in the corresponding tumor adjacent normal tissues from OSCC patients. Higher UBE2C expression was associated with poor cell differentiation and lymph node invasion in OSCC patients. High UBE2C expression was also correlated with shorter disease-specific survival in TSCC patients having poor cell differentiation, advanced pathological stages, lymph node metastasis as well as receiving radiation therapy. Compared to control cells, OSCC cells in which UBE2C was silenced showed decreased cell proliferation, migration/invasion and colony formation and they exhibited lower expression levels of the following cancer stemness markers—ALDH1/A2, CD44, CD166 and EpCAM. High co-expression levels of UBE2C/CD44, UBE2C/CD166 and UBE2C/EpCAM were associated with poor prognosis in oral cancer patients from The Cancer Genome Atlas database. Our findings indicated that UBE2C might be a potential biomarker for tumorigenesis and prognosis in TSCC.

【 授权许可】

Unknown