Beclin 1 Expression is Closely Linked to Colorectal Carcinogenesis and Distant Metastasis of Colorectal Carcinoma

International Journal of Molecular Sciences

Mei-Ying Zhang²  Wen-Feng Gou³  Shuang Zhao³  Xiao-Yun Mao¹  Zhi-Hong Zheng²  Yasuo Takano⁴ 

[1] Department of Surgical Oncology, the First Affiliated Hospital of China Medical University, Shenyang 110001, China; E-Mail:;Laboratory Animal Center, China Medical University, Shenyang 110001, China; E-Mails:;Department of Biochemistry and Molecular Biology, College of Basic Medicine, China Medical University, Shenyang 110001, China; E-Mails:;Clinical Research Institute, Kanagawa Cancer Center, Yokohama 241-0815, Japan; E-Mail:

关键词: colorectal carcinoma;    Beclin 1;    tumorigenesis;    pathogenesis;    prognosis;   

DOI  :  10.3390/ijms150814372

来源: mdpi

【 摘 要 】

Beclin 1 participates in development, autophagy, differentiation, anti- apoptosis, neurodegeneration, tumorigenesis and cancer progression. The roles of Beclin 1 in colorectal carcinogenesis and its subsequent progression are still unclear. Here, the mRNA and protein expression of Beclin 1 were determined in colorectal carcinoma and matched mucosa by Reverse transcriptase-polymerase chain reaction and Western blot. Immunohistochemistry and in situ hybridization (ISH) were performed on tissue microarryer with colorectal carcinoma, adenoma and mucosa. The expression of Beclin 1 mRNA and protein was found to be higher in colorectal carcinoma than matched mucosa by real-time PCR and Western blot (p < 0.05). According to the ISH data, Beclin 1 expression was lower in colorectal non-neoplastic mucosa (NNM) than adenoma and carcinoma (p < 0.05). Immunohistochemically, primary carcinoma showed stronger Beclin 1 expression than NNM and metastatic carcinoma in the liver (p < 0.05). Beclin 1 protein expression was negatively related to liver and distant metastasis (p < 0.05), but not correlated with age, sex, depth of invasion, lymphatic or venous invasion, lymph node metastasis, tumor-node-metastasis (TNM) staging, differentiation or serum carcinoembryonic antigen (CEA) concentration (p > 0.05). Survival analysis indicated that Beclin 1 expression was not linked to favorable prognosis of the patients with colorectal carcinoma (p > 0.05). Cox’s model indicated that depth of invasion and distant metastasis were independent prognostic factors for colorectal carcinomas (p < 0.05). It was suggested that Beclin 1 expression is closely linked to colorectal carcinogenesis and distant metastasis of colorectal carcinoma.

【 授权许可】

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© 2014 by the authors; licensee MDPI, Basel, Switzerland.