Bacterial Adhesion to Grade 4 and Grade 5 Turned and Mildly Acid-Etched Titanium Implant Surfaces: An In Vitro and Ex Vivo Study

Applied Sciences

Claudio Leone¹  Luca Sbricoli²  Riccardo Guazzo²  Luigi Guida³  Gennaro Cecoro³  Marco Annunziata³  Livia Nastri³ 

[1] Department of Engineering, University of Campania “Luigi Vanvitelli”, via Roma 29, 81031 Aversa (Caserta), Italy;Department of Neurosciences, School of Dentistry, University of Padova, Via Giustiniani 2, 35100 Padova, Italy;Multidisciplinary Department of Medical-Surgical and Dental Specialties, University of Campania “Luigi Vanvitelli”, Via L. De Crecchio 6, 80138 Naples, Italy;

关键词: dental implants;    bacterial adhesion;    surface properties;   

DOI  :  10.3390/app11167185

来源: DOAJ

【 摘 要 】

Aim of the present study was to investigate the bacterial adhesion to titanium (Ti) implant surfaces, different for composition and topographic features. Grade 4 and 5 turned (T-4, T-5,) and mildly acid-etched (MA-4, MA-5) Ti 6 × 1 mm disks were topographically analyzed by scanning electronic microscopy and 3D profilometry. Bacterial cultures (Streptococcus sanguinis) were in vitro seeded and, after two and six hours, adherent bacteria were quantified by colony-forming unit (CFU) counting. Ti samples were also exposed to the oral environment of six periodontally healthy volunteers and, after 12 h, the formed biofilm was evaluated by CFU counting. Inter-group differences were tested by the Mann–Whitney U-Test (α = 0.05). MA surfaces were significantly rougher than T ones, whereas no difference between grade 4 and grade 5 disks was detected. Significantly higher in vitro bacterial adhesion for MA than T disks was shown at two and six hours. Significantly higher values of CFU counting for MA than T surfaces and for grade 5 than grade 4 disks were found at the 12 h-ex vivo test. Bacterial adhesion showed to be sensitive to both Ti surface topography and composition, with possible implications on peri-implant tissue health maintenance.

【 授权许可】

Unknown