Biological Effect of Gamma Rays According to Exposure Time on Germination and Plant Growth in Wheat

Applied Sciences

Min Jeong Hong¹  Yeong Deuk Jo¹  Jin-Baek Kim¹  Sang Hoon Kim¹  Soon-Jae Kwon¹  Hong-Il Choi¹  Joon-Woo Ahn¹  Yong Weon Seo²  Dae Yeon Kim³ 

[1]Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, 29 Geumgu, Jeongeup 56212, Korea

[2]Division of Biotechnology, Korea University, 145 Anam-ro, Seongbuk-gu, Seoul 02841, Korea

[3]Institute of Animal Molecular Biotechnology, Korea University, 145 Anam-ro, Seongbuk-gu, Seoul 02841, Korea

关键词: wheat;    gamma rays;    exposure time;    free radicals;    growth inhibition;    mutation breeding;   

DOI  :  10.3390/app12063208

来源: DOAJ

【 摘 要 】

Gamma rays as a type of ionizing radiation constitute a physical mutagen that induces mutations and could be effectively used in plant breeding. To compare the effects of gamma and ionizing irradiation according to exposure time in common wheat (Keumgang, IT 213100), seeds were exposed to ⁶⁰Co gamma rays at different dose rates. To evaluate the amount of free radical content, we used electron spin resonance spectroscopy. Significantly more free radicals were generated in the case of long-term compared with short-term gamma-ray exposure at the same dose of radiation. Under short-term exposure, shoot and root lengths were slightly reduced compared with those of the controls, whereas long-term exposure caused severe growth inhibition. The expression of antioxidant-related and DNA-repair-related genes was significantly decreased under long-term gamma-ray exposure. Long-term exposure caused higher radiosensitivity than short-term exposure. The results of this study could help plant breeders select an effective mutagenic induction dose rate in wheat.

【 授权许可】

Unknown