Collateral sensitivity associated with antibiotic resistance plasmids

eLife

Jana Palkovičová¹  Ricardo León-Sampedro¹  Álvaro San Millán²  Jerónimo Rodríguez-Beltrán³  Cristina Herencias³  Aida Alonso-del Valle³  Rafael Cantón³ 

[1] Centro de Investigación Biológica en Red Epidemiología y Salud Pública, Instituto de Salud Carlos III, Madrid, Spain;Department of Biology and Wildlife Diseases, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic;Servicio de Microbiología. Hospital Universitario Ramón y Cajal and Instituto Ramón y Cajal de Investigación Sanitaria, Madrid, Spain;

关键词: antibiotics;    collateral sensitivity;    plasmid;   

DOI  :  10.7554/eLife.65130

来源: DOAJ

【 摘 要 】

Collateral sensitivity (CS) is a promising alternative approach to counteract the rising problem of antibiotic resistance (ABR). CS occurs when the acquisition of resistance to one antibiotic produces increased susceptibility to a second antibiotic. Recent studies have focused on CS strategies designed against ABR mediated by chromosomal mutations. However, one of the main drivers of ABR in clinically relevant bacteria is the horizontal transfer of ABR genes mediated by plasmids. Here, we report the first analysis of CS associated with the acquisition of complete ABR plasmids, including the clinically important carbapenem-resistance conjugative plasmid pOXA-48. In addition, we describe the conservation of CS in clinical E. coli isolates and its application to selectively kill plasmid-carrying bacteria. Our results provide new insights that establish the basis for developing CS-informed treatment strategies to combat plasmid-mediated ABR.

【 授权许可】

Unknown