Combined Sugarcane Pretreatment for the Generation of Ethanol and Value-Added Products

Frontiers in Energy Research

Daehwan Kim¹  Sidnei Emilio Bordignon²  Roberto da Silva²  Christiane da Costa Carreira Nunes²  Eleni Gomes²  Edivaldo Ximenes Ferreira Filho³  Michael R. Ladisch⁴  Eduardo Ximenes⁴  Olavo Micali Perrone⁵  Maurício Boscolo⁵ 

[1] Hodson Science and Technology Center, Department of Biology, Hood College, Frederick, Brazil;Laboratory of Biochemistry and Applied Microbiology, Department of Food Engineering and Science, UNESP—University Estadual Paulista—IBILCE, Sao José do Rio Preto, Brazil;Laboratory of Enzymology, Department of Cellular Biology, University of Brasília, Brasília, Brazil;Laboratory of Renewable Resources Engineering, Department of Agricultural and Biological Engineering, Purdue University, West Lafayette, IN, United States;Laboratory of Sucrochemistry and Analytical Chemistry, Department of Chemistry and Environmental Sciences, UNESP—University Estadual Paulista—IBILCE, Sao José do Rio Preto, Brazil;

关键词: ozone;    liquid hot water;    pretreatment;    enzyme hydrolysis;    ethanol fermentation;    value-added chemicals;   

DOI  :  10.3389/fenrg.2022.834966

来源: DOAJ

【 摘 要 】

In this work, we have tested individual and combination of applications of ozonolysis and liquid hot water (LHW) to pretreat sugarcane bagasse (SCB) for the removal of enzyme and/or microbial inhibitors and generation of potential value-added chemicals. A solid content with 80% cellulose and a liquid phase (liquor) rich in phenolic derived compounds (3 g.L−1) from lignin, sugars (>20 g.L−1), and other compounds, such as furfural and hydroxymethylfurfural (HMF), were generated. Maximal (59%) glucan conversion occurred in the presence of double-pretreated bagasse, which had 32–50% more glucan available than the samples that were individually LHW or ozone-pretreated, resulting in maximal ethanol production (92% after 42 h) from double-pretreated SCB enzyme hydrolyzate. In summary, this work showed that ozone reacts effectively with lignin without the use of any other chemical reagent, and LHW pretreatment, followed by a washing step, was effective in solubilizing and cleaning up the fiber enzyme and microbial inhibitory compounds with ozone being effective against phenolics. Moreover, the generated cellulose-rich substrate is readily fermentable. The acidic liquor fraction removed by sequential washings and containing mainly sugars and phenolic compounds may be evaluated for use in green chemistry bioconversions processes.

【 授权许可】

Unknown