Alterations in the Gut Microbiota of Zebrafish (Danio rerio) in Response to Water-Soluble Crude Oil Components and Its Mixture With a Chemical Dispersant

Frontiers in Public Health

Emanuel Hernández-Nuñez¹  Monica Améndola-Pimenta²  Rossanna Rodríguez-Canul²  Jesús Alejandro Zamora-Briseño²  Carlos Eduardo González-Penagos²  Juan Antonio Pérez-Vega²  Daniel Cerqueda-García² 

[1] CONACYT – Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Mérida, Mexico;Departamento de Recursos del Mar, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional-Unidad Mérida, Mérida, Mexico;

关键词: zebrafish;    gut microbiota;    crude oil;    WAF;    CEWAF;    dysbiosis;   

DOI  :  10.3389/fpubh.2020.584953

来源: DOAJ

【 摘 要 】

Crude oil spills have caused substantial impacts to aquatic ecosystems. Chemical dispersants are used to palliate the impact of oil spillages, but their use is polemic due to their additional potential toxic effect when mixed with oil-derived components. In this work, we used a 16S-based metagenomic approach to analyze the changes of the gut microbiota of adult zebrafish (Danio rerio) exposed to the water accommodated fraction (WAF) of a light crude oil (35° API gravity), and the chemically enhanced WAF (CEWAF), prepared with Nokomis 3-F4® dispersant. After 96 h of exposure, WAF induced an increase in the alpha and beta diversity, altering the relative abundance of Vibrio, Flavobacterium, and Novosphingobium. In contrast, CEWAF only caused an increase in the beta diversity, and an enrichment of the genus Pseudomona. Both treatments diminished the abundances of Aeromonas, Cetobacterium, Coxiella, Dinghuibacter, and Paucibacter. Moreover, the co-occurrence network among genera was more complex in WAF than in CEWAF, indicating a greater bacterial interaction in response to WAF. Our results indicate that short-term exposure to WAF and CEWAF can induce a dysbiosis in the gut microbiota of D. rerio, but these changes are specific in each treatment.

【 授权许可】

Unknown