Effect of incubation duration, growth temperature, and abiotic surface type on cell surface properties, adhesion and pathogenicity of biofilm-detached Staphylococcus aureus cells

AMB Express

Marwan Abdallah¹  Simon Oussama khelissa¹  Charafeddine Jama¹  Rabah Boukherroub²  Christine Faille³  Nour-Eddine Chihib³ 

[1] CNRS, ENSCL, UMR 8207-UMET-PSI, Université de Lille1;CNRS, IEMN, UMR 8520, Université de Lille1;CNRS, INRA, UMR 8207-UMET-PIHM, Université de Lille1;

关键词: Staphylococcus aureus;    Physiology;    Planktonic cells;    Biofilm-detached cells;    Surface properties;    Pathogenicity;   

DOI  :  10.1186/s13568-017-0492-0

来源: DOAJ

【 摘 要 】

Abstract The goal of this study was to investigate the effect of growth conditions such as the temperature (20, 30 and 37 °C), incubation duration (24 and 48 h) and surface type (stainless steel and polycarbonate) on the cell surface physicochemical properties and adhesion to abiotic surfaces of biofilm-detached and planktonic Staphylococcus aureus cells. This study tested also the hypothesis that S. aureus planktonic cells exhibit distinct pathogenic properties compared with their sessile counterparts. The results showed that the changes of the growth conditions promoted changes in the zeta potential, hydrophobicity, electron donor/acceptor character of the studied cell populations. Biofilm-detached cells showed a greater adhesion to stainless steel and polycarbonate compared with planktonic cells. Compared with planktonic cells, sessile ones showed higher cytotoxic effect against HeLa cells, DNase activity, and siderophore levels. The higher cytotoxic effect and production of DNase and siderophore increased with the increase of temperature and duration of incubations. Based on the obtained data, the S. aureus biofilm-detached cells were found to be distinct in many physiological properties compared with their planktonic counterparts.

【 授权许可】

Unknown