Enhanced Production of Anti-PD1 Antibody in CHO Cells through Transient Co-Transfection with Anti-Apoptotic Gene Bcl-xL Combined with Rapamycin

Processes

Huifang Zong¹  Jianwei Zhu¹  Chenxiao Xu¹  Lei Han¹  Xinyu Zhang¹  Xi Li²  Yunxia Li²  Lei Wang²  Jingyi Zhang²  Yuan Yuan²  Baohong Zhang² 

[1] Therapeutic Antibody, Ministry of Education, School of Pharmacy, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, China;;Engineering Research Center of Cell &

关键词: anti-apoptotic gene;    Bcl-x_L;    rapamycin;    Chinese Hamster Ovary;    transient gene expression technology;   

DOI  :  10.3390/pr7060329

来源: DOAJ

【 摘 要 】

CHO cells are often used to produce monoclonal antibodies in mammalian cell expression systems. In the process of large-scale cell culture, apoptosis is related to cell survival and product quality. Over-expressing an anti-apoptotic gene to delay apoptosis and improve cell growth is one of the strategies for improving productivity of monoclonal antibodies. Autophagy inducer rapamycin can extend the culture duration of CHO cells and affect the yield of antibodies. A method was developed for transient co-transfection of anti-apoptotic genes and genes of interest combined with rapamycin to increase the transient expression of the anti-PD1 antibody. Under the optimal transfection conditions, the combination of Bcl-x_L and rapamycin can significantly delay cell apoptosis, inhibit cell proliferation, and prolong cell life-time. As a result, anti-PD1 monoclonal antibody expression levels are increased by more than 2 times.

【 授权许可】

Unknown