Genomic sequencing and analysis of a Chinese hamster ovary cell line using Illumina sequencing technology

BMC Genomics

Research Article

Mihailo Kaplarevic¹  Stephanie Hammond²  Jeffrey C Swanberg²  Kelvin H Lee² 

[1] Delaware Biotechnology Institute, University of Delaware, 19711, Newark, DE, USA;Department of Chemical Engineering, University of Delaware, 19711, Newark, DE, USA;Delaware Biotechnology Institute, University of Delaware, 19711, Newark, DE, USA;

关键词: Chinese Hamster Ovary Cell;    Chinese Hamster Ovary;    Basic Local Alignment Search Tool;    Bacterial Artificial Chromosome Library;    Chinese Hamster Ovary Cell Line;   

DOI  :  10.1186/1471-2164-12-67

 received in 2010-09-14, accepted in 2011-01-26,  发布年份 2011

来源: Springer

【 摘 要 】

BackgroundChinese hamster ovary (CHO) cells are among the most widely used hosts for therapeutic protein production. Yet few genomic resources are available to aid in engineering high-producing cell lines.ResultsHigh-throughput Illumina sequencing was used to generate a 1x genomic coverage of an engineered CHO cell line expressing secreted alkaline phosphatase (SEAP). Reference-guided alignment and assembly produced 3.57 million contigs and CHO-specific sequence information for ~ 18,000 mouse and ~ 19,000 rat orthologous genes. The majority of these genes are involved in metabolic processes, cellular signaling, and transport and represent attractive targets for cell line engineering.ConclusionsThis demonstrates the applicability of next-generation sequencing technology and comparative genomic analysis in the development of CHO genomic resources.

【 授权许可】

Unknown   
© Hammond et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.