Comparison of Polysaccharides as Coatings for Quercetin-Loaded Liposomes (QLL) and Their Effect as Antioxidants on Radical Scavenging Activity

Polymers

Pedro Cruz-Alcantar¹  MaríaZenaida Saavedra-Leos¹  Alfredo Aguilar-Elguézabal²  Manuel Román-Aguirre³  César Leyva-Porras⁴ 

[1] Coordinación Académica Región Altiplano (COARA), Universidad Autónoma de San Luis Potosí, Matehuala 78700, Mexico;Departamento de Ingeniería y Química de Materiales, Centro de Investigación en Materiales Avanzados, S.C. (CIMAV), Chihuahua 31136, Mexico;Doctorado Institucional en Ingeniería y Ciencia de los Materiales, Universidad Autónoma de San Luis Potosí, San Luis Potosí 78210, Mexico;Laboratorio Nacional de Nanotecnología (NanoTech), Centro de Investigación en Materiales Avanzados S.C. (CIMAV), Chihuahua 31136, Mexico;

关键词: loaded liposomes;    antioxidant properties;    polysaccharides coating;    inulin;    lactose;    quercetin;   

DOI  :  10.3390/polym12122793

来源: DOAJ

【 摘 要 】

Liposomes are microstructures containing lipid and aqueous phases employed in the encapsulation and delivery of bioactive agents. Quercetin-loaded liposomes (QLLs) were coated with three different polysaccharides and then tested as radical scavengers. Lactose (LCQLL), chitosan (CCQLL), and inulin (ICQLL) were employed as coating materials. Particle size determined by light scattering, showed primary size of 200 nm for all samples, while a secondary particle size of 600 nm was observed for CCQLL. Scanning electron microscopy (SEM) evidenced particle aggregation with the addition of the polysaccharide coating. Transmission electron microscopy (TEM) revealed the layered microstructure of liposomes composed of at least two layers, and primary particle size below 100 nm. QLL showed higher antioxidant activity than the coated liposomes. This behavior was attributed to the chemical interaction between quercetin and the corresponding coating polysaccharide in the layered structure, which traps the quercetin and keeps it unavailable for radical scavenging. From the three polysaccharides, lactose showed a better performance as coating material in the antioxidant activity, which suggested that the smaller size of the disaccharide molecule resulted in a faster releasing of the quercetin in the solution. Thus, LCQLL is an advantageous way to deliver quercetin for antioxidant purposes, where the low stability in delivered media of quercetin loaded liposomes is commonly compromised.

【 授权许可】

Unknown