Droplet Microfluidics for the ex Vivo Expansion of Human Primary Multiple Myeloma Cells

Micromachines

Iciar Gonzalez¹  Pilar Carreras¹  Joaquin Martinez-Lopez²  Miguel Gallardo²  Alejandra Ortiz-Ruiz² 

[1] CSIC, Spanish National Research Council, 28006 Madrid, Spain;Hospital 12 Octubre, Hematology Department, Research institute i+12, 28040 Madrid, Spain;

关键词: microdroplets;    microfluidics;    stem cell encapsulation;   

DOI  :  10.3390/mi11030261

来源: DOAJ

【 摘 要 】

We previously reported a new approach for micromanipulation and encapsulation of human stem cells using a droplet-based microfluidic device We demonstrated the possibility of encapsulating and culturing difficult-to-preserve primary human hematopoietic stem cells using an engineered double layered bead composed by an inner layer of alginate and an outer layer of puramatrix constructed using a soft technology without the use of any external force. In this work, we use this micro manipulation technique to build a 3D scaffold as a biomimetic model to recapitulate the niche of patient-derived multiple myeloma cells (MM cell) using a multilayered 3D tissue scaffold constructed in a microfluidic device and cultured in 10% FBS culture medium. In the current study, we included the use of this biomimetic model comprising supporting human Mesenchymal stem cells to show the mid-term survival of MM cells in the proposed structures. We found that the generated microniches were suitable for the maintenance of MM cells with and without supporting cells. Additionally, cultured MM cells in droplets were exposed to both Bortezomib and Lenalidomide to test their toxicity in the cultured patient derived cells. Results indicate that the maintained MM cells were consistently responding to the applied medication, opening a wide field of possibilities to use the presented micro device as an ex vivo platform for drug screening.

【 授权许可】

Unknown