Molecular Mechanisms of Anti-Melanogenic Gedunin Derived from Neem Tree (Azadirachta indica) Using B16F10 Mouse Melanoma Cells and Early-Stage Zebrafish

Plants

Tae-Oh Kim¹  Hwang-Ju Jeon²  Kyeongnam Kim²  Sung-Eun Lee²  Myoung-Jin Kim²  Chaeeun Kim³ 

[1] College of Civil and Environmental Engineering, Kumoh National Institute of Technology, Gumi 39253, Korea;Department of Applied Biosciences, Kyungpook National University, Daegu 41566, Korea;Department of Integrative Biology Kyungpook National University, Daegu 41566, Korea;

关键词: MITF;    microphthalmia-associated transcription factor;    TYR;    tyrosinase;    DQ;    dopaquinone;   

DOI  :  10.3390/plants10020330

来源: DOAJ

【 摘 要 】

Melanogenesis represents a series of processes that produce melanin, a protective skin pigment (against ultraviolet rays), and determines human skin color. Chemicals reducing melanin production have always been in demand in the cosmetic market because of skincare interests, such as whitening. The main mechanism for inhibiting melanin production is the inhibition of tyrosinase (TYR), a key enzyme for melanogenesis. Here, we evaluated gedunin (Ged), a representative limonoid, for its anti-melanogenesis action. Melanin production in vitro was stimulated by alpha-melanocyte stimulating hormone (α-MSH) in B16F10 mouse melanoma cells. Ged reduced α-MSH-stimulated melanin production, inhibiting TYR activity and protein amount. We confirmed this result in vivo in a zebrafish model for melanogenesis. There was no sign of toxicity and malformation of zebrafish embryos during development in all treated concentrations. Ged reduced the number of produced zebrafish embryo pigment dots and melanin contents of embryos. The highly active concentration of Ged (100 µM) was much lower than the positive control, kojic acid (8 mM). Hence, Ged could be a fascinating candidate for anti-melanogenesis reagents.

【 授权许可】

Unknown