Kaliziri extract upregulates tyrosinase, TRP-1, TRP-2 and MITF expression in murine B16 melanoma cells

BMC Complementary and Alternative Medicine

Research Article

Aiden Eblimit¹  Maidina Kabas²  Haji Akber Aisa²  Yong-qiang Liu²  Ablajan Tulake³  Adila Tuerxuntayi³ 

[1] Human Genome Sequencing Center Department of Molecular and Human Genetics Baylor College of Medicine, 77030, Houston, TX, USA;The Key Laboratory of Plant Resources and Chemistry of Arid Zone, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, 830011, Urumqi, China;State Key Laboratory Basis of Xinjiang Indigenous Medicinal Plants Resource Utilization, 830011, Urumqi, China;The Key Laboratory of Plant Resources and Chemistry of Arid Zone, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, 830011, Urumqi, China;State Key Laboratory Basis of Xinjiang Indigenous Medicinal Plants Resource Utilization, 830011, Urumqi, China;University of the Chinese Academy of Sciences, 100039, Beijing, China;

关键词: KZE;    Melanogenesis;    B16 melanoma cells;    TYR;    MITF;   

DOI  :  10.1186/1472-6882-14-166

 received in 2013-12-11, accepted in 2014-05-12,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundKaliziri extract (KZE) is a traditional Uyghur medicine (TUM), used by traditional hospitals in China as an injection for treatment of vitiligo for more than 30 years. Clinical application has shown that this medicine has obvious therapeutic effects. However, its phytochemical analysis and mechanism have not been examined.MethodsKZE was extracted from seeds of Kaliziri [Vernonia anthelmintica (L.) Willd.] in ethanol-water (80:20, v/v), its components were identified by LC-MS/MS, and the signaling pathway of melanin synthesis in KZE treated murine B16 melanoma cells was examined by western blotting.ResultsLiquid chromatography-mass spectrometry analysis confirmed that the main components of KZE are flavonoids. KZE increased the tyrosinase activity and melanin content in a dose-dependent manner at concentrations of 5-40 μg/ml, and treatment with 20 μg/ml of KZE enhanced the expression of tyrosinase in B16 cells in a time-dependent manner.ConclusionsKZE induced melanogenesis by increasing the expression of TYR, TRP-1, TRP-2 and MITF in B16 cells.

【 授权许可】

Unknown   
© Tuerxuntayi et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.