The enantioselective study of the toxicity effects of chiral acetochlor in HepG2 cells

Ecotoxicology and Environmental Safety

Jufang Gao¹  Jingdi Zhang²  Wenping Xu³  Yang Zhang³  Susu Wang³  Liming Tao³ 

[1] College of Life, Shanghai Normal University, Shanghai 200234, China;Kao Chemical Corporation Shanghai, China;Shanghai Key Laboratory of Chemical Biology, School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China;

关键词: Acetochlor;    Enantiomers;    Cytotoxicity;    Apoptosis;    HepG2 cells;   

DOI  :  

来源: DOAJ

【 摘 要 】

Acetochlor is one of the most widely used chiral herbicides in the world, and it is usually produced and used as racemic form (Rac). The potential effects of acetochlor in human body are mainly induced by its residue in agriculture food. The direct target exposed is the liver in human body. However, the potential toxic and mechanism threat to human liver cells caused by chiral acetochlor has been rarely reported. The purpose of this study is to explore the potential mechanism of the toxicity caused by chiral acetochlor in HepG2 cells. The results revealed that acetochlor and its enantiomers could inhibit cell activity and cause DNA damage in HepG2 cells. The toxicity of Rac was higher than that of the two enantiomers, mainly derived from S configuration. The mechanism is through inducing decreased membrane potential (△Ψ), up-regulated Bax/BcL-2 expression, caused a cascade reaction, activated casepase-3 and casepase-9 and cleaved PARP, which maybe lead to cell death through apoptotic-signaling pathway in the end. These results illuminate that the genotoxic and cytotoxic risks of chiral acetochlor are major coming from S configuration. It provides a theoretical basis for the production of single pesticide to reduce the effects of human health.

【 授权许可】

Unknown