Dapagliflozin attenuates hypoxia/reoxygenation-caused cardiac dysfunction and oxidative damage through modulation of AMPK

Cell & Bioscience

Pei-Ling Hsieh¹  Shih-Hung Chan²  Wan-Ching Chou³  Hui-Ching Cheng³  Yu-Ting Huang³  Kun-Ling Tsai³ 

[1] Department of Anatomy, School of Medicine, China Medical University;Department of Internal Medicine, College of Medicine, National Cheng Kung University Hospital, National Cheng Kung University;Department of Physical Therapy, College of Medicine, National Cheng Kung University;

关键词: Dapagliflozin;    Ischemia/reperfusion injury;    AMPK;   

DOI  :  10.1186/s13578-021-00547-y

来源: DOAJ

【 摘 要 】

Abstract Background Emerging evidence demonstrated dapagliflozin (DAPA), a sodium-glucose cotransporter 2 inhibitor, prevented various cardiovascular events. However, the detailed mechanisms underlying its cardioprotective properties remained largely unknown. Results In the present study, we sought to investigate the effects of DAPA on the cardiac ischemia/reperfusion (I/R) injury. Results from in vitro experiments showed that DAPA induced the phosphorylation of AMPK, resulting in the downregulation of PKC in the cardiac myoblast H9c2 cells following hypoxia/reoxygenation (H/R) condition. We demonstrated that DAPA treatment diminished the H/R-elicited oxidative stress via the AMPK/ PKC/ NADPH oxidase pathway. In addition, DAPA prevented the H/R-induced abnormality of PGC-1α expression, mitochondrial membrane potential, and mitochondrial DNA copy number through AMPK/ PKC/ NADPH oxidase signaling. Besides, DAPA reversed the H/R-induced apoptosis. Furthermore, we demonstrated that DAPA improved the I/R-induced cardiac dysfunction by echocardiography and abrogated the I/R-elicited apoptosis in the myocardium of rats. Also, the administration of DAPA mitigated the production of myocardial infarction markers. Conclusions In conclusion, our data suggested that DAPA treatment holds the potential to ameliorate the I/R-elicited oxidative stress and the following cardiac apoptosis via modulation of AMPK, which attenuates the cardiac dysfunction caused by I/R injury.

【 授权许可】

Unknown