Gamma Aminobutyric Acid-Enriched Fermented Oyster (Crassostrea gigas) Increases the Length of the Growth Plate on the Proximal Tibia Bone in Sprague-Dawley Rats

Molecules

SuHyun Hong¹  DaHye Kim¹  Hyun Hwangbo¹  MinYeong Kim¹  YungHyun Choi¹  SeonYeong Ji¹  SoYoung Kim¹  Hyesook Lee¹  Gi-Young Kim²  You-Jin Jeon²  SuJeong Lee³  Freshet Assefa³  EuiKyun Park³  Joung-Hyun Park⁴  Bae-Jin Lee⁴ 

[1] Anti-Aging Research Center, Dong-eui University, Busan 47340, Korea;Department of Marine Life Science, Jeju National University, Jeju 63243, Korea;Department of Pathology and Regenerative Medicine, School of Dentistry, Kyungpook National University, Daegu 41940, Korea;Ocean Fisheries & Biology Center, Marine Bioprocess Co., Ltd., Busan 46048, Korea;

关键词: fermented oyster;    gamma aminobutyric acid;    insulin like growth factor-1;    recombinant human growth hormone;    tibial growth plate;   

DOI  :  10.3390/molecules25194375

来源: DOAJ

【 摘 要 】

Bone growth during childhood and puberty determines an adult’s final stature. Although several prior studies have reported that fermented oyster (FO) consisting of a high amount of gamma aminobutyric acid can be attributed to bone health, there is no research on the efficacy of FO on growth regulation and the proximal tibial growth plate. Therefore, in this study, we investigated the effect of FO oral administration on hepatic and serum growth regulator levels and the development of the proximal tibial growth plate in young Sprague-Dawley rats. Both oral administration of FO (FO 100, 100 mg/kg FO and FO 200, 200 mg/kg FO) and subcutaneous injection of recombinant human growth hormone (rhGH, 200 μg/kg of rhGH) for two weeks showed no toxicity. Circulating levels of growth hormone (GH) significantly increased in the FO 200 group. The expression and secretion of insulin-like growth factor-1 (IGF-1) and insulin-like growth factor binding protein-3 (IGFBP-3) were enhanced by FO administration. FO administration promoted the expression of bone morphogenic proteins IGF-1 and IGFBP-3 in the proximal tibial growth plate. This positive effect of FO resulted in incremental growth of the entire plate length by expanding the proliferating and hypertrophic zones in the proximal tibial growth plate. Collectively, our results suggested that oral administration of FO is beneficial for bone health, which may ultimately result in increased height.

【 授权许可】

Unknown