Detecting Early-Stage Oral Cancer from Clinically Diagnosed Oral Potentially Malignant Disorders by DNA Methylation Profile

Cancers

Kazuki Mori¹  Hiroshi Hijioka¹  Kenichi Kume¹  Yumiko Mishima¹  Tsuyoshi Sugiura¹  Mahiro Beppu¹  Hiroki Tsuchihashi¹  Yuichi Goto¹  Keitaro Nishi¹  Tomofumi Hamada² 

[1] Department of Maxillofacial Diagnostic and Surgical Science, Field of Oral and Maxillofacial Rehabilitation, Graduate School of Medical and Dental Sciences, Kagoshima University, Kagoshima 890-8544, Japan;Department of Oral & Maxillofacial Surgery, Hakuaikai Social Medical Corporation, Sagara Hospital, Kagoshima 892-0833, Japan;

关键词: oral cancer;    oral squamous carcinoma;    oral potentially malignant disorders;    gargle fluid;    DNA methylation;    biomarker;   

DOI  :  10.3390/cancers14112646

来源: DOAJ

【 摘 要 】

Clinically, early-stage oral cancers are difficult to distinguish from oral potentially malignant disorders (OPMDs), and invasive tissue biopsy should be performed to determine a treatment strategy. Previously, we focused on gargle fluid as a noninvasive testing method and reported aberrant methylation in gargle fluid in patients with oral cancer. This study aimed to distinguish early-stage oral cancer from clinically diagnosed OPMDs using gargle fluid samples. We collected gargle fluid samples from 40 patients who were clinically diagnosed with OPMDs in the training set; among them, 9 patients were pathologically diagnosed with oral cancer. Methylation levels of 25 tumor suppressor genes were analyzed using the methylation-specific multiplex ligation-dependent probe amplification (MS-MLPA) method. We found that a combination of six genes (TP73, CASP8, RARB, KLLN, GSTP1, and CHFR) could distinguish oral cancer from clinically diagnosed OPMDs with high diagnostic performance (area under the curve [AUC], 0.885; sensitivity, 77.8%; and specificity, 87.1%). Additionally, the panel comprised of the six methylated genes was validated in the test set. Furthermore, when compared with cytology testing, the panel could accurately detect oral cancer. The present methylated gene panel may serve as a novel biomarker for oral cancer.

【 授权许可】

Unknown