Potential enhancement of host immunity and anti-tumor efficacy of nanoscale curcumin and resveratrol in colorectal cancers by modulated electro- hyperthermia

BMC Cancer

Albert T. C. Liao¹  Chen-Si Lin¹  I-Ming Kuo¹  Chiao-Hsu Ke¹  Jih-Jong Lee²  Yu-Shan Wang³  Winston Han⁴  Pei-Jong Hsieh⁴  Cheng-Chung Huang⁴  Hsin-Chien Chiang⁴ 

[1] Department of Veterinary Medicine, School of Veterinary Medicine, National Taiwan University;Graduate Institute of Veterinary Clinical Science, School of Veterinary Medicine, National Taiwan University;Institute of Molecular Medicine and Bioengineering, National Chiao Tung University;JohnPro Biotech Inc.;

关键词: Modulated electro-hyperthermia (mEHT);    Curcumin;    Resveratrol;    Nanosized;    Apoptosis;    Tumor microenvironment;   

DOI  :  10.1186/s12885-020-07072-0

来源: DOAJ

【 摘 要 】

Abstract Background Modulated electro-hyperthermia (mEHT) is a form of hyperthermia used in cancer treatment. mEHT has demonstrated the ability to activate host immunity by inducing the release of heat shock proteins, triggering apoptosis, and destroying the integrity of cell membranes to enhance cellular uptake of chemo-drugs in tumor cells. Both curcumin and resveratrol are phytochemicals that function as effective antioxidants, immune activators, and potential inhibitors of tumor development. However, poor bioavailability is a major obstacle for use in clinical cancer treatment. Methods This purpose of this study was to investigate whether mEHT can increase anti-cancer efficacy of nanosized curcumin and resveratrol in in vitro and in vivo models. The in vitro study included cell proliferation assay, cell cycle, and apoptosis analysis. Serum concentration was analyzed for the absorption of curcumin and resveratrol in SD rat model. The in vivo CT26/BALB/c animal tumor model was used for validating the safety, tumor growth curve, and immune cell infiltration within tumor tissues after combined mEHT/curcumin/resveratrol treatment. Results The results indicate co-treatment of mEHT with nano-curcumin and resveratrol significantly induced cell cycle arrest and apoptosis of CT26 cells. The serum concentrations of curcumin and resveratrol were significantly elevated when mEHT was applied. The combination also inhibited the growth of CT26 colon cancer by inducing apoptosis and HSP70 expression of tumor cells while recruiting CD3+ T-cells and F4/80+ macrophages. Conclusions The results of this study have suggested that this natural, non-toxic compound can be an effective anti-tumor strategy for clinical cancer therapy. mEHT can enable cellular uptake of potential anti-tumor materials and create a favorable tumor microenvironment for an immunological chain reaction that improves the success of combined treatments of curcumin and resveratrol.

【 授权许可】

Unknown