Optimization and Validation of a New Microbial Inhibition Test for the Detection of Antimicrobial Residues in Living Animals Intended for Human Consumption

Foods

Luis Mata¹  Alejandra Antón¹  Pedro Razquin¹  Rafael Pagán²  María Jesús Serrano²  Santiago Condón²  Diego García-Gonzalo² 

[1] Department of R&D, ZEULAB S.L., 50197 Zaragoza, Spain;Instituto Agroalimentario de Aragón-IA2, Universidad de Zaragoza-CITA, 50013 Zaragoza, Spain;

关键词: antibiotics;    sulfonamides;    antimicrobials;    blood;    in vivo;    biological test;   

DOI  :  10.3390/foods10081897

来源: DOAJ

【 摘 要 】

Even though antibiotics are necessary in livestock production, they can be harmful not only due to their toxicity, but also in view of their contribution to the emergence of antimicrobial resistance. Screening tests based on microbial growth inhibition appeared to be useful tools to prevent its entry into the food chain. They have nevertheless been traditionally carried out post mortem, leading to great economical loss and harm to the environment in case a positive sample is found. Hence, the objective was to evaluate the use of a screening test as an ante mortem alternative for the detection of antibiotic residues in meat: thus, Explorer^®-Blood test was optimized and validated. After adapting the procedure for matrix preparation, the assay parameters were assessed from 344 antibiotic-free blood serum samples. Limits of Detection (LoDs) were defined by spiking blood serum with several of the most common antimicrobials used in veterinary practice. LoDs were similar to those obtained for meat and were at or below the maximum residue limits set by EU legislation for muscle. Analyses of in vivo injected samples, previously characterized by LC-MS/MS, demonstrated the method’s accuracy and proved that Explorer^®-Blood can be considered a suitable alternative to conventional post mortem screening methods.

【 授权许可】

Unknown