Multiresidue determination of antibiotics in preserved eggs using a QuEChERS-based procedure by ultrahigh-performance liquid chromatography tandem mass spectrometry

Acta Chromatographicae

Wen Sheng¹  Chen Ziliang²  Li Yonggang³ 

[1] Department of Biotechnology, Delft University of Technology, Delft 2624BC;Hubei Provincial Key Laboratory for Applied Toxicology, Hubei Provincial Center for Disease Control and Prevention Wuhan 430079;School of Environment, Beijing Normal University, State Key Laboratory of Water Environment Simulation/Key Laboratory of Water and Sediment Sciences of Ministry of Education, Beijing 100875

关键词: QuEChERS;    UHPLC–MS/MS;    antibiotics;    macrolides;    quinolones;    sulfonamides;    preserved eggs;   

DOI  :  10.1556/1326.2017.29211

学科分类：化学（综合）

来源: Akademiai Kiado Rt.

【 摘 要 】

A modified QuEChERS (quick, easy, cheap, effective, rugged, and safe) method and ultrahigh-performance liquid chromatography coupled to tandem mass spectrometry (UHPLC–MS/MS) were optimized and validated for 16 antibiotics belonging to three families (macrolides, quinolones, and sulfonamides) that were found in preserved eggs. Samples were extracted in 4 mL water and 10 mL acetonitrile with 1% acetic acid and subjected to a cleanup procedure using dispersive solid-phase extraction with C18 and primary secondary amine sorbents, prior to detection by UHPLC–MS/MS. Matrix-matched calibration was used for quantification to reduce the matrix effect with limits of quantification in the range of 0.3–3.0 μg/kg. Validation of the method was conducted by recovery and precision experiments. Recoveries of the spiked samples ranged from 73.8% to 127.4%, and the intra- and inter-day relative standard deviations were lower than 21.2% and 22.3%, respectively. This method was successfully applied to the analysis of antibiotics in preserved egg samples.

【 授权许可】

Unknown