Chemical Composition and Protective Effect of Young Barley (Hordeum vulgare L.) Dietary Supplements Extracts on UV-Treated Human Skin Fibroblasts in In Vitro Studies

Antioxidants

Sylwia Katarzyna Naliwajko¹  Anna Puścion-Jakubik¹  Krystyna Joanna Gromkowska-Kępka¹  Joanna Bielecka¹  Monika Grabia¹  Renata Markiewicz-Żukowska¹  Justyna Moskwa¹  Patryk Nowakowski¹  Konrad Mielcarek¹  Katarzyna Socha¹  Jolanta Soroczyńska¹ 

[1] Department of Bromatology, Faculty of Pharmacy with the Division of Laboratory Medicine, Medical University of Białystok, Mickiewicza 2D Str., 15-222 Białystok, Poland;

关键词: young barley;    antioxidant properties;    UV radiation;    skin care;    nutricosmetics;    fibroblasts;   

DOI  :  10.3390/antiox10091402

来源: DOAJ

【 摘 要 】

Young barley seems to be a promising material for use as nutricosmetic due to the presence of many biologically active compounds. The aim of this study was to evaluate the effect of Hordeum vulgare L. extracts on human skin fibroblasts exposed to ultraviolet radiation B (UVB) radiation. Analysis of the chemical composition showed a predominance of 9,12,15-octadecatrienoic acid. The quality assessment showed that young barley preparations have high total polyphenolic content (TPC) and favourable total antioxidant status (TAS). They also contain antioxidant elements such as zinc, copper, and selenium. Furthermore, the analyzed products were found to be safe in terms of toxic elements (lead, cadmium and mercury) and lack of cytotoxic effect of young barley extracts on cells. In vitro bioactivity assays showed that young barley extract increased the survival rate and accelerated the migration of fibroblasts in research models with UVB radiation. The application of both extracts caused an increase in DNA biosynthesis, and in the number of cells arrested in S phase. Moreover, an inhibitory effect of the tested extracts on the expression of matrix metalloproteinase 2 (MMP-2) and matrix metalloproteinase 9 (MMP-9) was observed. The results indicate that young barley extracts, due to protective as well as restorative effect, could potentially be used in the production of nutricosmetics and skin care products.

【 授权许可】

Unknown