Integrated Transcript and Metabolite Profiles Reveal That EbCHI Plays an Important Role in Scutellarin Accumulation in Erigeron breviscapus Hairy Roots

Frontiers in Plant Science

Jian Yu¹  Xing Xiang²  Ruibing Chen²  Hexin Tan²  Yichao Fang²  Lei Zhang²  Baokang Huang³  Xianghui Chen³  Qing Li⁴  Shouhong Gao⁴  Tingting Zhu⁴  Wansheng Chen⁴  Jianghua Liu⁶ 

[1] College of Life Science, Fujian Agriculture and Forestry University, Fuzhou, China;Department of Pharmaceutical Botany, School of Pharmacy, Second Military Medical University, Shanghai, China;Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, Shanghai, China;Department of Pharmacy, Changzheng Hospital, Second Military Medical University, Shanghai, China;Development and Utilization Key Laboratory of Northeast Plant Materials, School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang, China;School of Forestry, Southwest Forestry University, Kunming, China;

关键词: Erigeron breviscapus;    correlation analysis;    scutellarin;    chalcone isomerase;    hairy root culture;   

DOI  :  10.3389/fpls.2018.00789

来源: DOAJ

【 摘 要 】

Scutellarin, a flavonoid 7-O-glucuronide, is an essential bioactive compound of Erigeron breviscapus (Vaniot) Hand.-Mazz. used for the treatment of cerebrovascular diseases. However, due to overexploitation and overuse, E. breviscapus is facing the problems of extinction and habitat degradation. In this study, a correlation analysis between the transcript and metabolite profiles of methyl jasmonate (MeJA)-treated E. breviscapus at different time points indicated that chalcone isomerase (EbCHI) was the primary contributor to scutellarin accumulation during flavonoid biosynthesis. EbCHI was then further characterized as a chalcone isomerase that efficiently converted chalcone to naringenin in vitro. Optimal parameters derived by comparing different culture conditions were successfully used to establish hairy root cultures of E. breviscapus with a maximum transformation rate of 60% in B5 medium. Furthermore, overexpression of EbCHI significantly enhanced scutellarin accumulation in E. breviscapus hairy roots with a maximum content of 2.21 mg g-1 (dw), 10-fold higher than that of natural roots (0.21 mg g-1 dw). This study sheds new light on a method of effective gene-based metabolic engineering by accurate and appropriate strategies and provides a protocol for hairy root cultures that accumulate high levels of scutellarin, providing a promising prospect for relieving the overexploitation and unavailability of E. breviscapus in the future.

【 授权许可】

Unknown