Simultaneous determination of three active constituents in Erigeron breviscapus by high-performance liquid chromatography

Dynamic Chiropractic

关键词: high-performance liquid chromatography (HPLC);    Erigeron breviscapus;    scutellarin;    1;    5-dicaffeoylquinic acid;    1;    3-dicaffeoylquinic acid.;   

DOI  :  10.5897/JMPR11.1285

学科分类：医学（综合）

来源: MPA Media

【 摘 要 】

A rapid, simple and reliable high-performance liquid chromatographic (HPLC) method was developed for simultaneous determination of compound 1 (scutellarin), compound 2 [1,5-dicaffeoylquinic acid (1,5-DQA)] and compound 3 [1,3-dicaffeoylquinic acid (1,3-DQA)] inErigeron breviscapus. The three constituents were measured on a Zorbax XDB-C18column (4.6 mm × 150 mm, 5 μm) with a gradient elution of acetonitrile (0.5%) aqueous phosphoric acid at wavelength of 335 nm and a flow rate of 1.0 ml/min. Linearity of each standard was established in the concentration range of 19.375 to 310.0 μg/ml for compound 1, 6.125 to 98.0 μg/ml for compound 2, and 3.875 to 62.0 μg/ml for compound 3, respectively, with correlation coefficientr> 0.9990. Average recoveries (n= 6) of compounds 1 to 3 were 100.3% with a relative standard deviations (RSD) of 1.5%, 98.9% with a RSD of 1.9%, 98.6% with a RSD of 2.0%, respectively. The proposed method was successfully applied to simultaneously determinethe three active constituents inE. breviscapusfrom different habitats and germplasm resources for the first time.

【 授权许可】

Unknown