The Vital Dye CDr10b Labels the Zebrafish Mid-Intestine and Lumen

Molecules

Shantanu Patke¹  Young-Tae Chang¹  Alan J. Davidson²  Jung Y. Lee³  Veronika Sander³ 

[1] Pathology, University of Auckland, Auckland 1142, New Zealand;;Department of Chemistry &;Department of Molecular Medicine &

关键词: zebrafish;    gut development;    mid-intestine;    CDr10b;    vital dye;    laser ablation;   

DOI  :  10.3390/molecules22030454

来源: DOAJ

【 摘 要 】

We describe the use of the fluorescent reporter compound CDr10b to label mid-intestinal structures in zebrafish larvae after simple immersion. CDr10b is deposited into the gut where it initially fills the lumen and is excreted. Using laser-mediated injury of the intestine, we show that CDr10b provides a useful readout of the integrity and repair of the epithelial cell barrier. In addition, CDr10b specifically labels the absorptive mid-intestine segment that is analogous to the mammalian small intestine. By perturbing retinoic acid signaling, which regulates the size of the mid-intestine segment, we show that CDr10b is a valuable tool to rapidly assess developmental malformations of the intestine in live animals.

【 授权许可】

Unknown