Journal of Functional Foods | |
Lactobacillus casei JY300-8 generated by 12C6+ beams mutagenesis inhibits tumor progression by modulating the gut microbiota in mice | |
Qiaoqiao Li1  Junkai Wang1  Jin Bai1  Guoqing Xiao2  Shuyang Wang3  Fuqiang Xu3  Miaoyin Dong3  | |
[1] College of Nuclear Science and Technology, University of Chinese Academy of Sciences, Beijing, PR China;Institute of Biology, Gansu Academy of Sciences, Lanzhou, Gansu, PR China;Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou, Gansu, PR China; | |
关键词: Probiotic; Functional foods; Lactobacillus casei; Heavy ion beams induced-mutagenesis; Microbiota; Colon cancer; | |
DOI : | |
来源: DOAJ |
【 摘 要 】
Probiotics, as novel functional foods via microbiota-modulation to enhance host health and inhibit tumor carcinogenesis, have been attracted considerable interests. L. casei JY, as a potential probiotic, was irradiated by 12C6+ beams and a new mutant strain JY300-8 was generated through three round selections of transit tolerance in the upper human gastrointestinal tract, adhesion ability to Caco-2 cells and inhibitory pathogenic microorganisms, respectively. Interestingly, JY300-8 presented excellent properties, including the strongest adhesion ability (86.81%) to Caco-2 cells to our knowledge and more excellent antimicrobial properties, which was used for the potential candidate probiotic. Additionally, JY300-8 could significantly inhibit the proliferation of Caco-2, HT29 and HCT116 cells in vitro, respectively. Furthermore, there were better therapeutic effects at an early stage in colon cancer mice, which included the similar survival rates (90%) and stronger inhibitory effect on tumor (83.48%), lower treatment cost and less side effects in comparison to the therapy drug (DFUR) for colon cancer (65.65%), which was attributed to promoting the apoptosis of colon cells and enhancing antitumor response via modulation cancer-induced gut microbiota dysbiosis by oral administration of JY300-8. Therefore, JY300-8 has great potential for bio-prophylactic and bio-therapeutic functional food in colon carcinogenesis. This work provided a promising approach that could improve human health, reduce greatly cancer incidence and mitigate cancer progression via modulation gut microbiota by excellent probiotics intervention.
【 授权许可】
Unknown