Altered expression of miR-29a-3p and miR-34a-5p by specific inhibition of GSK3β in the MPP+ treated SH-SY5Y Parkinson's model

Non-coding RNA Research

Claude Bernard¹  Shahsanam Abbasi²  Mojtaba Ahmadzadeh-Darinsoo³  Azita Parvaneh Tafreshi³  Morteza Ahmadzadeh-Darinsoo³  Ehsan Arefian⁴ 

[1] Australian Regenerative Medicine Institute, Monash University, Melbourne, 3800, Victoria, Australia;Department of Agricultural Biotechnology, National Research Institute of Genetic Engineering and Biotechnology, Tehran, Iran;Department of Medical Biotechnology, National Research Institute of Genetic Engineering and Biotechnology, Tehran, Iran;School of Biology, College of Science, University of Tehran, Tehran, Iran;

关键词: Parkinson's disease;    Neuroprotection;    SH-SY5Y;    7-BIO;    MPP+;    miR-29a;   

DOI  :  

来源: DOAJ

【 摘 要 】

In the current study, the effects of 7-BIO as a specific GSK3β inhibitor was examined on cell survival and expression of miR-29a-3p and miR-34a-5p in neurotoxin MPP+ treated SH-SY5Y cells. Our findings revealed that while co-treatment of the cells with 7-BIO and MPP+ did not alter the toxicity induced by MPP+, pretreatment with 3.5 μM 7-BIO for 6 h increased the survival of the 2 mM MPP+ treated cells. Also, qRT-PCR analysis of gene expression showed that while miR-29a-3p was unchanged in cells treated with either 2 mM MPP+ or 3.5 μM 7-BIO alone, miR-34a-5p was increased by MPP+ but decreased by 7-BIO. Pretreatment with 3.5 μM 7-BIO prior to MPP+ however, increased miR-29a-3p but decreased miR-34a-5p induced by MPP+. We therefore suggest that 7-BIO inhibition of GSK3β alleviates the MPP+ induced neurotoxicity by regulating miR-29a-3p and miR-34a-5p expressions in Parkinson's disease model SH-SY5Y cells.

【 授权许可】

Unknown