High-frequency submicrosecond electroporator

Biotechnology & Biotechnological Equipment

Jurgita Švedienė¹  Algimantas Paškevičius¹  Audrius Grainys²  Vitalij Novickij²  Paulius Butkus²  Sonata Tolvaišienė²  Jurij Novickij² 

[1] Laboratory of Biodeterioration Research, Nature Research Centre, Institute of Botany;Vilnius Gediminas Technical University;

关键词: electroporation;    mosfet;    crowbar;    electro-permeabilization;    nanosecond pulse;    electric field;   

DOI  :  10.1080/13102818.2016.1150792

来源: DOAJ

【 摘 要 】

In this work, we present a novel electroporator which is capable of generating single and bursts of high power (3 kV, 60 A) square wave pulses of variable duration (100 ns to 1 ms) with predefined repetition frequency (1 Hz to 3.5 MHz). The proposed synchronized crowbar implementation ensures a constant pulse rise and fall times, which are independent from the load, thus highly relevant in electroporation. The electroporator was successfully tested for the inactivation of the human pathogen Candida albicans. The device is compatible with standard commercial electroporation cuvettes.

【 授权许可】

Unknown