Detection of the Plasmodium falciparum Kelch-13 gene P553L mutation in sporozoites isolated from mosquito salivary glands in South-Central Vietnam

Parasites & Vectors

Shusuke Nakazawa¹  Yoshimasa Maeno²  Kaoru Hori²  Gaku Masuda³  Nguyen Tuyen Quang⁴  Ron P. Marchand⁴  Satoru Kawai⁵  Richard Culleton⁶ 

[1]Department of Protozoology, Institute of Tropical Medicine, Nagasaki University

[2]Department of Virology and Parasitology, Fujita Health University School of Medicine

[3]Faculty of International Liberal Arts, Juntendo University

[4]Khanh Phu Malaria Research Unit, Medical Committee Netherlands-Viet Nam

[5]Laboratory of Tropical Medicine and Parasitology, Dokkyo Medical University

[6]Malaria Unit, Department of Pathology, Institute of Tropical Medicine, Nagasaki University

关键词: Plasmodium falciparum;    Anopheles dirus;    Sporozoite;    Artemisinin resistance;    K13-propeller gene;    Vietnam;   

DOI  :  10.1186/s13071-017-2247-9

来源: DOAJ

【 摘 要 】

Abstract Background Plasmodium falciparum has developed resistance against artemisinin in Southeast Asia. Mutations in the P. falciparum Kelch-13 (Pfk13) gene are associated with artemisinin resistance in vitro and in vivo. We investigated the prevalence of mutations in PfK13 from sporozoite-stage parasites isolated from the salivary glands of Anopheles dirus mosquitoes. Methods Mosquitoes were caught by human-landing catches at two locations within the Khanh Phu commune, South-Central Vietnam. Identification of Anopheles species was performed based on morphological features and nucleotide sequence analysis. Sporozoite-infected salivary glands were stored on filter paper and at 4–6 °C. A nested-PCR targeting the small subunit ribosomal RNA gene was used for Plasmodium species identification. Pfk13 was amplified by nested PCR, and subjected to nucleotide sequencing. Results Five of 33 P. falciparum sporozoite samples carried the P553L mutation at the PfK13 locus. This mutation has been recorded previously in Vietnam, but not in Khanh Hoa province, were surveys of K13 polymorphism have not previously been carried out. Conclusion These results demonstrate the utility of mosquito-stage malaria parasite samples for studies on the molecular epidemiology of drug resistance.

【 授权许可】

Unknown