Why Is the Correct Selection of Trichoderma Strains Important? The Case of Wheat Endophytic Strains of T. harzianum and T. simmonsii

Journal of Fungi

H. Camilo Insuasti¹  Enrique Monte¹  María Illescas¹  Rosa Hermosa¹  Theodora Neagu¹  M. Belén Rubio¹  Alberto Pedrero-Méndez¹ 

[1] Institute for Agribiotechnology Research (CIALE), Department of Microbiology and Genetics, University of Salamanca, Campus de Villamayor, C/Duero, 12, 37185 Salamanca, Spain;

关键词: T. afroharzianum;    biological control agent (BCA);    Fusarium graminearum;    ACC deaminase;    fungal phytohormones;    IAA;   

DOI  :  10.3390/jof7121087

来源: DOAJ

【 摘 要 】

The search for endophytic fungi in the roots of healthy wheat plants from a non-irrigation field trial allowed us to select 4 out of a total of 54 cultivable isolates belonging to the genus Trichoderma, identified as T. harzianum T136 and T139, T. simmonsii T137, and T. afroharzianum T138. In vitro assays against the phytopathogenic fungus Fusarium graminearum showed that the T. harzianum strains had the highest biocontrol potential and that T136 exhibited the highest cellulase and chitinase activities. Production patterns of eight phytohormones varied among the Trichoderma strains. All four, when applied alone or in combination, colonized roots of other wheat cultivars and promoted seed germination, tillering, and plant growth under optimal irrigation conditions in the greenhouse. Apart from T136, the endophytic Trichoderma strains showed plant protection capacity against drought as they activated the antioxidant enzyme machinery of the wheat plants. However, T. simmonsii T137 gave the best plant size and spike weight performance in water-stressed plants at the end of the crop. This trait correlated with significantly increased production of indole acetic acid and abscisic acid and increased 1-aminocyclopropane-1-carboxylic acid deaminase activity by T137. This study shows the potential of Trichoderma endophytes and that their success in agricultural systems requires careful selection of suitable strains.

【 授权许可】

Unknown