Mice Plasmacytoid Dendritic Cells Were Activated by Lipopolysaccharides Through Toll-Like Receptor 4/Myeloid Differentiation Factor 2

Frontiers in Immunology

Jun-O Jin¹  Juyoung Hwang¹  Eun-Koung An¹  Wei Zhang³ 

[1] Department of Medical Biotechnology, Yeungnam University, Gyeongsan, South Korea;Research Institute of Cell Culture, Yeungnam University, Gyeongsan, South Korea;Shanghai Public Health Clinical Center, Shanghai Medical College, Fudan University, Shanghai, China;

关键词: lipopolysaccharide;    plasmacytoid dendritic cell;    conventional dendritic cell;    toll-like receptor 4;    myeloid differentiation factor 2;   

DOI  :  10.3389/fimmu.2021.727161

来源: DOAJ

【 摘 要 】

Plasmacytoid dendritic cells (pDCs) are known to respond to viral infections. However, the activation of pDCs by bacterial components such as lipopolysaccharides (LPS) has not been well studied. Here, we found that pDCs, conventional dendritic cells (cDCs), and B cells express high levels of toll-like receptor 4 (TLR4), a receptor for LPS. Moreover, LPS could effectively bind to not only cDCs but also pDCs and B cells. Intraperitoneal administration of LPS promoted activation of splenic pDCs and cDCs. LPS treatment led to upregulation of interferon regulatory factor 7 (IRF7) and induced production of interferon-alpha (IFN-α) in splenic pDCs. Furthermore, LPS-dependent upregulation of co-stimulatory molecules in pDCs did not require the assistance of other immune cells, such as cDCs. However, the production levels of IFN-α were decreased in cDC-depleted splenocytes, indicating that cDCs may contribute to the enhancement of IFN-α production in pDCs. Finally, we showed that activation of pDCs by LPS requires the TLR4 and myeloid differentiation factor 2 (MD2) signaling pathways. Thus, these results demonstrate that the gram-negative component LPS can directly stimulate pDCs via TLR4/MD2 stimulation in mice.

【 授权许可】

Unknown