In Vitro and In Vivo Effects of Ketamine on Generation and Function of Dendritic Cells

Journal of Pharmacological Sciences

Jingxian Zeng¹  Shuxuan Xia¹  Wa Zhong²  Liling Lin¹  Jie Li¹ 

[1] Department of Anesthesiology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, China;Department of Gastroenterology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, China

关键词: anesthesia;    ketamine;    dendritic cell;    plasmacytoid dendritic cell;    conventional dendritic cell;   

DOI  :  10.1254/jphs.11113FP

学科分类：药学

来源: Nihon Yakuri Gakkai Henshuubu / Japanese Pharmacological Society

【 摘 要 】

References(49)Cited-By(6)The question about how intravenous anesthetic reagents affect the development and function of dendritic cell subsets still has no comprehensive answers. Bone marrow cells differentiated with FMS-like tyrosine kinase 3 ligand in vitro represented the steady-state dendritic cell subsets. The effects of ketamine on the generation and function of dendritic cell subsets were investigated. We found that dendritic cell subsets responded to the anesthetic reagent ketamine in several aspects: 1) The in vitro and in vivo development of plasmacytoid dendritic cells were inhibited by ketamine at high concentrations; 2) The endocytosis of dendritic cells were not influenced by ketamine at concentrations from 50 – 200 μM; 3) The maturation markers of conventional dendritic cells were not changed by ketamine upon LPS or CpG stimulation, although the cytokines mRNA profiles were affected; 4) The allogenic-stimulatory activity of dendritic cells was suppressed by ketamine. In conclusion, ketamine hampered plasmacytoid dendritic cell subset development both in vivo and in vitro. The dendritic cells maturation and downstream responses towards different toll-like receptor stimuli were differently regulated by ketamine treatment.

【 授权许可】

Unknown