Adhesive Bacteria in the Terminal Ileum of Children Correlates With Increasing Th17 Cell Activation

Frontiers in Pharmacology

Charlie Xiang¹  Mizu Jiang²  Kerong Peng²  Xiaoli Shu²  Diya Ye²  Bo Chen²  Weizhong Gu²  Lingling Luo²  Weirong Liu³  Xiaojun Wang³ 

[1] Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China;Gastrointestinal Lab, Children’s Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Child, National Children's Regional Medical Center, Hangzhou, China;Shaoxing People’s Hospital, Shaoxing, China;

关键词: Th17 cells;    adhesive bacteria;    terminal ileum;    Crohn’s disease;    SIgA;   

DOI  :  10.3389/fphar.2020.588560

来源: DOAJ

【 摘 要 】

Humans and symbiotic bacteria are interdependent and co-evolved for millions of years. These bacteria communicate with human hosts in the gut in a contact-independent metabolite. Because most intestinal bacteria are non-adhesive, they do not penetrate the mucus layer and are not directly in contact with epithelial cells (ECs). Here, we found that there are adhesive bacteria attached to the Children's terminal ileum. And we compared the immune factors of non-adhesive bacteria in the children ileum with adhesive bacteria as well. Stimulated Th17 cell associated with adherent bacteria in the ileum ECs. SIgA responses are similar to those roles in mouse experiments. Immunohistochemical analysis confirmed that the expression of SAA1, IL-2, IL-17A, foxp3, RORγt, TGFβ, and protein increased in Th17 cells. Finally, we used 16S rRNA genes 454 pyrosequencing to analyze the differences in bacterial communities between adhesive and non-adhesive bacteria in the ileum. Ileum with adherent bacteria demonstrated increased mucosa-related bacteria, such as Clostridium, Ruminococcus, Veillonella, Butyricimonas, and Prevotella. We believe that adhesive bacteria in children’s terminal ileum associated with an increased Th17 cell activation and luminal secretory IgA. Adhesive bacteria very closely adhere to terminal ileum of children. They may play important role in human gut immunity and Crohn’s disease.

【 授权许可】

Unknown